奥曲肽对中分化胃腺癌细胞的生长调控  

Effect of OCT on growth of human moderately-differentiated gastric cancer cell line in vitro

作　　者：马晓燕[2] 张南征[1] 

机构地区：[1]中国人民解放军第九七医院消化内科，江苏徐州221004 [2]徐州医学院肿瘤学教研室，江苏徐州221002

出　　处：《徐州医学院学报》2003年第3期225-228,共4页Acta Academiae Medicinae Xuzhou

基　　金：江苏省教育厅自然科学研究基金资助课题 (0 2KJD32 0 0 34)

摘　　要：目的研究生长抑素类似物奥曲肽(OCT)对人胃癌细胞株SGC7901的体外调控作用及其机制，并探讨奥曲肽与5-FU是否具有协同作用。方法采用MTT比色分析法测定奥曲肽和5-FU对细胞生长的调控；采用流式细胞术分析OCT 10^-6mol／L对SGC7901细胞周期分布的影响。结果OCT 10^-5mol／L、10^-6mol／L和5-FU及两药联合对SGC7901细胞生长均有抑制作用，OCT 10^-5mol／L的抑制率为32．36％；OCT 10^-6 mol／L与5-FU联合的抑制率(37．56％)高于两药单独使用(5-FU31．01％；10^-6 mol／L OCT 13．50％)。OCT 10^-6 mol／L可诱导SGC7901细胞G0/G1阻滞，于加药后12h开始，24h最显著(71．29％±1．25％，P<0．01 vs相应对照组)，36h消失；而24h换液、换药组36h时GD幅阻滞依然存在，48h也消失。G0/G1阻滞的同时伴S期细胞比例减少；亚二倍体细胞比例未见显著改变。结论OCT可以抑制体外培养的人胃癌细胞株SGC7901的生长，其机制之一是诱导细胞G0/G1期阻滞。OCT和5-FU协同抑制SGC7901细胞生长。Objective To study the effects of somatostatin analogue (octreotide, OCT) on the growth of human gastric cancer cell line SGC7901 and its synergism with 5-FU. Methods MTT assay was used to evaluate the cell proliferation of SGC7901 cell line, and the cell cycle distribution was measured by flow cytometric analysis. Results Both 5-FU and OCT inhibited the growth of SGC7901 cells, OCT of 10 -5 mol/L produced a maximum inhibitory rate of 32.36% (P<0.01 vs control). 5-FU enhanced the action of OCT of 10 -6 mol/L, producing an inhibition of 37.56%, higher than 5-FU and OCT alone (P<0.05 and P<0.001 respectively). OCT of 10 -6 mol/L also arrested SGC7901 cells at G 0/G 1 phase and reduced the proportion of S-phase cells, which began 12 h after the addition of OCT, became marked at 24 h and disappeared at 36 h. Conclusion OCT inhibits the growth of SGC7901 cells, and one of inhibitory mechanisms is the G 0/G 1 phase arrest. OCT and 5-FU are synergestic in action.

关 键 词：生长抑素类似物 胃肿瘤 癌细胞 奥曲肽 5-FU 

分 类 号：R735.2[医药卫生—肿瘤]