γ-连环素、c-Myc在常见类型肺癌中的表达及相关研究  

Expression of γ-catenin,c-Myc in Common Lung Cancer

作　　者：刘红云[1] 吴翠环[1] 吴人亮[1] 周晟[1] 

机构地区：[1]华中科技大学同济医学院基础医学院病理学系,武汉430030

出　　处：《华中科技大学学报（医学版）》2003年第4期351-353,457,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目 (No .39870 30 6 )

摘　　要：目的　探讨γ 连环素 (γ cat)、原癌蛋白c Myc与常见类型肺癌临床病理学特性之间的关系。 方法　应用免疫组织化学方法和原位分子杂交技术检测了常见类型肺癌中γ cat、γ catmRNA和c Myc的表达变化。 结果　常见类型肺癌中γ cat和c Myc的异常表达率分别为 6 1 6 7%、 5 3 33%。γ cat异常表达与肿瘤的分化程度及淋巴结转移有关 (P <0 0 5 ) ,c Myc过度表达与肿瘤体积大小及淋巴结转移有关 (P <0 0 5 )。γ cat异位表达与c Myc过度表达显著相关 (rs=0 35 0 ;P =0 0 0 6 )。 38例标本中γ catmRNA异常表达率为 6 3 16 % ,与肿瘤组织学类型、分化程度、淋巴结转移均相关 (P <0 0 5 )。结论　检测γ cat、c Myc在常见类型肺癌中的表达变化是判断肿瘤恶性程度。Objective To investigate the relationship between γ catenin, c Myc protein and the characteristics of common lung cancer. Methods By using immunohistochemical stainning and in situ hybridization techniques, the expression of γ catenin, γ catenin mRNA and c Myc in common lung cancer was detected respectively in 60 cases of paraffin wax sections and 38 cases of frozen sections. Results Immunohistochemistry results showed that among the 60 cases the abnormal expression rates of γ catenin and c Myc were 61 67 % and 53 33 % respectively. The aberrant expression of γ catenin was related to the differentiation and lymph node metastasis ( P <0 05). The overexpression of c Myc was related to the tumor size and lymph node metastasis ( P <0 05). Moreover, there was a close correlation between the abnormal size expression of γ catenin and the overexpression of c Myc ( r s=0 350, P=0 006 ). Among 38 cases of frozen sections the number of reduced expression of γ catenin mRNA was 24. And the reduced expression of γ catenin mRNA was related to histology types, differentation and lymph node metastasis ( P <0 05). Conclusion Detection of the variation of expression of γ catenin, c Myc in common lung carcinomas is very useful to evaluate the differentiation and metastasis.

关 键 词：Γ-连环素 原癌基因蛋白质 C-MYC 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]