原发性肝细胞癌中5种细胞周期蛋白的表达及其临床意义  

Immunohistochemical demonstration of cyclins A, B, D1, D3 and E in hepatocellular carcinomas using tissue microarrays

作　　者：朱明华[1] 倪灿荣[1] 祝峙[1] 李芳梅[1] 张顺民[1] 

机构地区：[1]第二军医大学长海医院病理科,上海200433

出　　处：《中华病理学杂志》2003年第5期440-443,共4页Chinese Journal of Pathology

基　　金：国家自然科学基金资助 ( 30 0 70 839)

摘　　要：目的　探讨原发性肝细胞癌中 5种细胞周期蛋白 (cyclin)的表达及其与肝癌细胞的增殖状态、侵袭转移和乙型肝炎病毒 (HBV )感染的相关性。方法　应用Instrumedics公司生产的组织芯片制作仪 ,将 2 73例原发性肝癌组织、14 4例癌旁肝组织和 10例尸检非肝病死亡肝组织制成组织芯片 ,取样针直径 2 .0mm ,采用免疫组织化学方法分别检测了cyclinA、cyclinB、cyclinD1、cyclinD3及cyclinE在肝癌、癌旁肝及尸检肝组织的表达率 ,并分析了肝癌、癌旁肝组织中HBV感染与这 5种cyclin表达间的相关性。 结果　共获得 3个肝癌组织芯片蜡块 ,分别含 13 6、14 3和 14 8个位点。在2 73例肝癌组织标本中 5种cyclin的阳性表达率分别为cyclinA 52 7%、cyclinB 45 4%、cyclinD13 5 9%、cyclinD3 4 4 3 %和cyclinE 2 3 1% ;在 14 4例癌旁肝组织中分别为 8 3 %、5 6%、4 9%、6 3 %和 1 4% ;10例尸检肝组织除 1例cyclinD1阳性外 ,其余均为阴性。 5种cyclin在肝癌组织中的阳性表达率均显著高于癌旁肝组织 (P <0 0 1) ;组织学分级为Ⅱ、Ⅲ级的肝癌组织 5种cyclin的表达高于Ⅰ级 (P <0 0 5) ;除cyclinA外 ,伴有门静脉癌栓组的阳性率高于无癌栓组 (P <0 0 1) ;HBV感染与cyclin的表达无明显相关性 (P >0 0 5)。结论　各个cyclin?Objective To investigate the expression of five kinds of cyclins in hepatocellular carcinoma (HCC) and their association with degree of tumor differentiation, metastasis and infection of hepatitis B virus (HBV). Methods The HCC tissue microarrays were composed of those from 273 cases of HCC tissues, 144 surrounding-tumor liver tissues and 10 normal liver tissues obtained from autopsy. The diameter of each specimens on tissue microarrays was 2.0 mm. Immunohistochemistry was used to detect the expression of cyclin A, cyclin B, cyclin D1, cyclin D3 and cyclin E on HCC tissue microarrays. The association of the expression of these cyclins with the infection rate of HBV was also analyzed. Results Three paraffin- embedded HCC tissue microarrays were successfully constructed, i ncluding 136, 143 and 148 tissue spots, respectively. The positive rates of cyclins in 273 cases of HCC were cyclin A 52.7%, cyclin B 45.4%, cyclin D1 35.9%, cyclin D3 44.3% and cyclin E 23.1%; while the figures in 144 surrounding-tumor tissues were 8.3%, 5.6%, 4.9%, 6.3% and 1.4%, respectively. In 10 normal liver tissues these cyclins exhibited negative staining, with the exception that cyclin D1 was positive in one case of normal liver tissue. The positive rate of cyclins in HCC were significant higher than those in surrounding-tumor liver tissues (P<0.01), in HCC tissues with histological grade Ⅱ and Ⅲ,the cyclins expression were stronger than that in grade Ⅰ (P<0.05). The positive rates of cyclins, except cyclin A in HCC with portal vein invasion were higher than those without portal vein invasion (P<0.01). Infection of HBV did not have significant relationship with the expression of cyclins (P>0.05). Conclusion Cyclins in different cell cycles overexpressed at varied levels in hepatocellular carcinoma, and the increased expression of cyclins may shorten the tumor cell cycle phase, accelerate cell proliferation, and have a close relationship with HCC aggressiveness.

关 键 词：高表达 癌旁肝组织 肝癌组织 肝癌细胞 原发性肝细胞癌 HBV感染 细胞周期蛋白 表达率 标本 组织芯片 

分 类 号：R735.7[医药卫生—肿瘤] R737[医药卫生—临床医学]