水稻Ds插入纯合体的筛选和鉴定  

Selection and Identification of Ds Insertion Homozygotes in Rice (Oryza sativa L.)

作　　者：陈兆贵[1] 王江[2] 张泽民[1] 刘芳[1] 朱海涛[1] 宛新杉[2] 张景六[2] 张桂权[1] 

机构地区：[1]华南农业大学农学院 [2]中国科学院上海生命科学研究院植物生理生态研究所

出　　处：《植物生理与分子生物学学报》2003年第4期337-341,共5页Journal Of Plant Physiology and Molecular Biology

基　　金：国家重点基础研究规划项目 (G19990 116)资助

摘　　要：采用Basta抗性鉴定、潮霉素抗性鉴定和PCR检测相结合的方法筛选和鉴定了水稻Ds插入纯合体。在T1代 2 36个转化株系中 ,有 16个株系的全部植株表现出对Basta的敏感 ,其余 2 2 0个株系的植株表现出对Basta的抗性。经过 3代的纯合筛选 ,共鉴定出Ds插入纯合体 2 0 3个 ,这些Ds插入纯合体可用于构建Ac/Ds系统和对Ds插入突变体进行筛选和鉴定 ,为水稻功能基因组学研究提供了材料。Transposon tagging is a useful approach for gene isolation in higher plant.Its first step is the development of Ac/Ds system. In this paper, the identification of the homozygotes with Ds insertion in rice ( Oryza sativa L.) by a method combining Basta resistance, hygromycin resistance and PCR polymorphism (Fig.1) is described. A total of 236 transformed lines were subjected to Ds homozygote selection.In T 1 generation, 220 lines were found to be resistant to Basta and 16 lines were susceptible (Table 1). After two generations of selfing of the 220 Basta-resistant lines, 203 Ds homozygotes were identified and the other 17 lines were still segregating (Tables 2, 3). The Ds homozygotes can be used to develop Ac/Ds system and to detect mutants for rice functional genomic study.

关 键 词：转座子 Ds插入突变 水稻 

分 类 号：S511.03[农业科学—作物学]