人基因组YAC分子克隆库的构建及DMD基因YAC克隆的筛选  

The Construction of Human Genome YAC Library and Screening by Hybridization of Membrane High Density Spoted in Array

作　　者：柴建华[1] 顾杨洪[1] 

机构地区：[1]复旦大学遗传学研究所,上海200433

出　　处：《Acta Genetica Sinica》1993年第4期285-289,共5页

摘　　要：以pYAC4为载体,以正常人白细胞和含4条X染色体的细胞株GM1414为DNA源构建成人基因组YAC(Yeast Artificial Chromosome,酵母人工染色体)分子克隆库,已得到原始克隆近2万个,插入DNA片段长度在400—1000kb,从其中选出一组YAC克隆,它们含有DMD基因全部DNA顺序。The human genomic YAC (Yeast Artificial Chromosome) library was constructed with human white blood cell and the cell line GM1414 containing 4 X chromosome DNA using pYAC 4 as vector. Twenty thousand YAC clones were obtained with insert size of 400-1000kb. A set of YACs containing entire DMD geneDNA sequence were selected.

关 键 词：酵母 人工染色体 人类 基因组 

分 类 号：Q75[生物学—分子生物学]