运动训练对心理应激机体肿瘤坏死因子α的影响  

Effect of physical exercise on tumor necrosis factor alpha after psychological stress

作　　者：郭庆秋[1] 颜军[1] 孙开宏[1] 

机构地区：[1]扬州大学体育学院体育系,江苏省扬州市225009

出　　处：《中国临床康复》2005年第8期142-143,共2页Chinese Journal of Clinical Rehabilitation

基　　金：江苏省教育厅自然科学基金研究指导性项目(IK0310064)~~

摘　　要：目的:心理应激因素可通过影响中枢神经和内分泌系统来改变机体免疫功能,而适当的应激干预手段可以改变应激对免疫机能的影响。通过对肿瘤坏死因子α(tumornecrosisfactorα,TNF-α)的检测,探讨运动训练对心理应激干预的免疫学机制。方法:实验于2003-09/2003-11在扬州大学体育学院体育心理学实验室完成,实验室为清洁级。健康雄性SD大鼠46只,体质量(240±30)g,SPF级,购于中科院上海动物研究中心许可证号:SCXK(沪)2003-0004。随机分为6组,即对照组、心理应激组、30min运动组、应激+30min运动组、60min运动组、应激+60min运动组(除30min运动组和应激+30min运动组每组7只外,其余每组各8只),分笼饲养,每笼3～4只;自然昼夜节律光照,饲养笼选用塑料制品,并配有不锈钢、塑料吸水瓶和铝吸水管、饲养温度15～16℃,国家标准固体混合饲料,自由饮食。结果:①经过2周的心理应激后,大鼠产生显著的身心变化,血清TNF-α的含量显著下降。②经过8周的运动训练后,大鼠血清TNF-α含量没有变化;同时,应激+30min运动组(1.451±0.188)μg/L和应激+60min运动组(1.696±0.246)μg/L的TNF-α的含量均显著高于应激组(1.293±0.166)μg/L,其中60min运动组最为明显(P<0.01)。结论:小运动负荷在正常状态下对机体免疫促进作用有限。AIM:The body's immune function can be influenced by psychological stress through the changes of the central nervous system and neuroendocrine system.However,the suitable means of intervention can change the effect of psychological stress on the immune function.Based on the previous researches,this article is to detect the tumor necrosis factor α(TNF-α) so as to evaluate the possible immunological mechanism of physical exercise intervening to psychological stress. METHODS:The experience was conducted from September to November 2003 in laboratory for psychology,College of Physical Education,Yangzhou University.Forty-six healthy male SD rats(SPF) with the body mass of (240±30) g bought from Shanghai Research Center for Experimental Center of Chinese Academy of Sciences[crtification number of SCXK(Shanghai) 2003-0004] were divided into six groups.The 6 groups were control group(n=8),psychological stress group(n=8),30-minute physical exercise group(n=7),psychological stress combined with 30-minute physical exercise group(n=7),60-minute physical exercise group(n=8)and phychological stress combined with 60-minute physical exercise(n=8).The rats were raised in separate cages with 3 or 4 rats in each cage.Natural and rhythmic light was shined day and night.The cages for breeding were plastic with many stainless steel and plastic bottles,and aluminum drinking tubes.The temperature for breeding was at 15 to 16 ℃.The feed were standard solid mixture made in China for eating and drinking freely. RESULTS:①After 2-week psychological stress,the rats had significant psychosomatic changes,and the level of serum TNF-αwas significantly decreased.②After eight-week physical exercise,the level of serum TNF-αwas not changed.It was(1.451±0.188) μg/L in 30-minute physical exercise group and (1.696±0.246) μg/L in 60-minute physical exercise group,both significantly higher than that in the psychological stress group[(1.293±0.166) μg/L],the most significant in the 60-minute physical exercise group(P< 0.01). CONCLUSION:The

关 键 词：运动 心理学 应激 免疫抑制 肿瘤坏死因子 

分 类 号：R749[医药卫生—神经病学与精神病学]