不同培养基和激素浓度处理对草地早熟禾愈伤组织诱导的影响  

Effect of Different Culture Medium and Hormone Concentration on Callus Induction of Poa Pratensis L.

作　　者：段碧华[1] 韩宝平[1] 高遐虹[1] 尹伟伦[2] 

机构地区：[1]北京农学院植物科技系,102206 [2]北京林业大学生物学院,100083

出　　处：《中国农学通报》2005年第2期24-27,共4页Chinese Agricultural Science Bulletin

基　　金：北京市科技新星项目资助(H020821300120)。

摘　　要：以3种不同的草地早熟禾种子为外植体,研究了不同培养基、不同浓度的2,4-D、BA及水解酪蛋白对愈伤组织诱导的影响。试验表明:(1)MN6培育基有利于愈伤组织的诱导;(2)种胚愈伤组织的诱导和继代宜采用MN6+2,4-D3mg/L+BA0.2mg/L的培养基;(3)水解酪蛋白对草地早熟禾种子愈伤组织诱导的影响不大;(4)3种草地早熟禾种子中,新哥莱德(Nuglade)出愈率较高。Study was carried out on effect of different concentration acetic acid(2,4-D),6-benzylamino purine(6-BA)and casein hydroly-sate(Hy)under different culture medium on callus induction of three varieties of Poa Pratensis L.with its seeds as explants.The result showed that (1)MN6 culture medium could improve callus induction rate;(2)culture medium of callus induction and regeneration of embryo was MN6 +2,4-D 3mg/L+BA0.2mg/L;(3)effect of different concentration casein hydroly-sate(Hy) was weak on callus induction;(4) callus induction rate of Nuglade was the highest in three varieties of Poa Pratensis L.

关 键 词：草地早熟禾 愈伤组织诱导 培养基 激素浓度 种子 BA 出愈率 影响 处理 培育基 

分 类 号：S688.4[农业科学—观赏园艺] S567[农业科学—园艺学]