帕金森病大鼠模型激发自体脑内神经干细胞增殖的实验研究  

Experimental study on the proliferation of neural stem cells of rat model of Parkinson's disease

作　　者：王磊[1] 曾水林[1] 李涛[1] 朱建宝[1] 雷志年[1] 韩洋[1] 

机构地区：[1]东南大学基础医学院解剖与组胚学系,江苏南京210009

出　　处：《现代医学》2004年第6期356-359,共4页Modern Medical Journal

基　　金：江苏省自然科学基金资助项目 (BK 2 0 0 2 0 56)

摘　　要：目的　研究帕金森病 (Parkinson’sdisease ,PD)大鼠激发自体脑内神经干细胞的增殖情况。方法　选用SD大鼠 ,将 6 羟多巴胺 (6 OHDA)注入纹状体内制作PD大鼠模型 ,假手术对照组注入等量生理盐水 ,于不同时间点处死大鼠。处死前均注射 5 溴脱氧尿苷 (Brdu)。免疫组织化学方法动态检测Brdu和巢蛋白 (Nestin)的表达 ,以确定脑内增殖细胞和神经前体细胞数量 ,Brdu/Nestin免疫双标确定脑内神经干细胞的增殖情况。结果　同正常组、假手术对照组比较 ,PD大鼠损伤侧海马区、侧脑室室管膜下区和黑质区的Brdu+ 细胞、Nestin+ 细胞和Brdu+ /Nestin+ 细胞数在模型成功后的第 3、5、7天明显增加 ,14d后逐渐减少 ,2 8d后恢复到正常水平。结论　 6 OHDA纹状体内注射制作PD的模型大鼠能够激活自体神经干细胞增殖。Objective To explore the proliferation of neural stem cells in the rat model of Parkinson's disease.Methods Rat model of Parkinson's disease was made by injecting the 6-hydroxydopamine (6?OHDA) into the striatum. The control group was made by injecting saline into the same site. The rats were killed at different time points and their brain were taken out. Before they were killed,the rats were injected Brdu into their abdomen. The dynamic expression of Nestin and Brdu were determined by immunohistochemical staining.Brdu labeling method was used to mark the cells of proliferation.Nestin expression was used to identify neuroprogenitor cells. And they both used to mark the diving neural stem cells.Results Campared with the controls, the number of Nestin-positive cells,Brdu-positive cells, and Brdu-Nestin-positive cells increased strikingly in the hippocampus,subventricular zone and substantia nigra at d 3,5,7, then declined at d 14 and almost reached the normal after 28 d. Conclusion The rat model of Parkinson's disease by direct injection of 6?OHDA in the striatum can stimulates the proliferation of inherent neural stem cells.

关 键 词：脑内 自体 PD 大鼠模型 神经干细胞增殖 帕金森病 6-OHDA 激发 情况 结论 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R338[医药卫生—临床医学]