氦氖激光对体外培养神经元生长发育的影响  

Influence of He-Ne laser irrigation on the growth and development of neurons cu ltured in vitro

作　　者：张顺清[1] 吴爱群[2] 张伟宏[1] 岳新灿[1] 曾光伟[1] 

机构地区：[1]郑州大学基础医学院人体解剖学教研室,河南省郑州市450052 [2]解放军第二军医大学人体解剖学教研室,上海市200433

出　　处：《中国临床康复》2005年第1期88-90,i002,共4页Chinese Journal of Clinical Rehabilitation

基　　金：河南省科技攻关项目(02110438)~~

摘　　要：目的:研究氦氖激光照射对神经元的影响。方法:采用无血清培养的新生大鼠海马神经元和额前叶皮质神经元,观察氦氖激光照射对神经元生长发育的影响。结果:氦氖激光照射能促进体外培养神经元的神经突起生长,增加神经元胞体面积及直径,提高神经元存活率。用Nissle染色法分析证明,氦氖激光照射能明显增加神经元内氏体的含量。结论:氦氖激光照射对神经元生长发育有重要促进作用。AIM: To study the influence of He Ne laser irrigation on neurons. METHODS: The influence of He Ne laser irrigation on the growth and developmen t of neurons in hippocampus and cortex of frontal lobe neurons was observed in t he cell culture of serum free medium. RESULTS: The number of survival cells and the outgrowth of neuritis were incre ased by He Ne laser irrigation in cultured neurons .In the presence of He Ne l aser irrigation,the diameter and area of neurons as well as the number of Nissl e bodies in the cells were markedly increased with image analysis and Nissle sta ining. CONCLUSION: The He Ne laser irrigation plays an important role in the growth and development of neurons.

关 键 词：大脑皮质 神经元 氦 氖 激光 

分 类 号：R743[医药卫生—神经病学与精神病学]