局灶性脑缺血大鼠脑保护作用中阿司匹林预处理及其最佳剂量选择  

Pretreatment of aspirin and selection of optimal dosage in cerebral protection o f rats with focal cerebral ischemia

作　　者：王倩[1] 孙晓晶[1] 季占胜[1] 闵连秋[1] 

机构地区：[1]锦州医学院附属第一医院神经内科,辽宁省锦州市121001

出　　处：《中国临床康复》2005年第1期108-109,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨阿司匹林预处理对缺血性脑损伤的保护机制及其最佳剂量。方法:实验于2004-06/07在锦州医学院药理学实验室完成。选用健康雄性SD大鼠,随机分为假手术组、缺血对照组、阿司匹林预处理组。阿司匹林预处理组又分为5,15,45,150mg/kg4个剂量组。以线栓法阻塞大脑中动脉制作脑缺血模型。24h后进行神经功能评分并断头取脑制备组织匀浆测诱导型一氧化氮合酶(iNOS)活性。结果:阿司匹林5mg/kg组、15mg/kg组和45mg/kg组的神经功能评分分别为(0.67±0.39),(0.83±0.75),(1.50±0.24)分,缺血对照组为(2.50±1.05)分(P<0.01),iNOS活性分别为0.84±0.54),(0.91(±0.43),(1.06±0.27)U/mg,缺血对照组为1.54±0.56)U/mg((P<0.01)。阿司匹林150mg/kg组在神经功能评分及iNOS活性方面分别为(2.17±1.17)分,(1.56±0.75)U/mg,与缺血对照组差别无显著性意义(P>0.05)。结论:小剂量阿司匹林预处理对随后的缺血性脑损伤具有保护作用,其机制可能是抑制iNOS活性,最佳剂量为5mg/kg。AIM:To investigate the pretreatment of aspirin on the protective mechanism and the optimal dosage of ischemic cerebral injury. METHODS:The experiment was completed in the Laboratory of Pharmacology,Jinzhou Medical College from June to July in 2004.The healthy male SD rats were randoml y divided into sham operation group,ischemic control group and aspirin pretreat ment group.Then the aspirin pretreatment group was also divided into 4 subgroups ,including 5 mg/kg group,15 mg/kg group,45 mg/kg group and 150 mg/kg group.The i schemic model was established with line embolism to block the middle cerebral ar tery.After 24 hours,the neurologic score was performed,and the activity of induc ed nitricoxide synthasethe(iNOS) was assayed after the establishment of tissues homogenate with decapitation and brain extraction. RESULTS:The neurological scores were(0.67±0.39) scores in 5 mg/kg aspirin gro up,(0.83±0.75) scores in 15 mg/kg aspirin group and(1.50±0.24) scores in 45 mg /kg aspirin group,and that in ischemic control group was(2.50±1.05) scores(P< 0 .01).The activity of iNOS was(0.84±0.54) u,(0.91±0.43) u and(1.06±0.27) u res pectively,and that in ischemic control group was(1.54±0.56) u,(P< 0.01).In 150 mg/kg aspirin group, the neurological score and the activity of iNOS were(2.17± 1.17) scores and(1.56±0.75) u,respectively,which was no significant difference compared with those of ischemic control group(P >0.05). CONCLUSION:The pretreatment of micro dosage aspirin can protect the subsequen t ischemic cerebral injury.Its mechanism is the inhibition of iNOS activity, and the optimal dosage is 5 mg/kg.

关 键 词：阿司匹林 缺血预处理 脑损伤 一氧化氮合酶 

分 类 号：R743[医药卫生—神经病学与精神病学]