白纹伊蚊唾液抗血小板凝集因子Apyrase基因片段的克隆测序  

PART SEQUENCE OF APYRASE GENE OF THE VECTOR MOSQUITO, AEDES ALBOPICTUS

作　　者：朱礼华[1] 赵彤言[1] 董言德[1] 陆宝麟 

机构地区：[1]军事医学科学院微生物流行病研究所全军媒介生物学重点实验室,北京100071

出　　处：《寄生虫与医学昆虫学报》2001年第4期227-231,共5页Acta Parasitologica et Medica Entomologica Sinica

基　　金：中国人民解放军总后勤部"九五"杰出青年基金资助项目

摘　　要：抗血小板凝集因子Apyrase是吸血昆虫唾液的重要功能因子 ,能水解被吸血宿主血液中血小板释放的ATP、ADP而抑制血小板凝集 ,以利吸血过程的顺利进行。本研究针对我国的白纹伊蚊 ,用兼并引物PCR法扩增了抗血小板凝集因子Apyrase的基因片断 ,并克隆入T载体进行了测序 ,经Clastal比较发现与埃及伊蚊相应序列有很高的同源性 ,为进一步的研究打下基础。The siliva of hematophagous insects contains a variety of pharmacologically active substances that counteract the normal hemostatic response to injury invertebrate hosts. Apyrase inhibits ADP dependent platelet aggregation. Here, degenerate oligo nucleotide primers based on its conserved amino acid sequence were used to amplify from genomic DNA of Aedes albopictus, an apyrase gene fragment was obtained. Sequence comparisons showed high similarity with Aedes aegypti, a member of 5′ nucleotidase super family.

关 键 词：抗血小板凝集 白纹伊蚊 唾液 e基因 埃及伊蚊 吸血昆虫 PCR法 T载体 克隆测序 片段 

分 类 号：R346[医药卫生—基础医学]