高压氧对链脲佐菌素大鼠糖尿病性心肌病变的治疗作用  

Role of Hyperbaric Oxygen on Diabetic Cardiomyopathy in Streptozocin Rats

作　　者：周亚刚[1] 刘芳[2] 邵贵强[3] 张杰[4] 周景花[3] 钟慈声[5] 施劲东[1] 许剑锋[1] 卜国强[3] 姚庆姑[2] 

机构地区：[1]复旦大学附属上海市第五人民医院心内科 [2]复旦大学附属上海市第五人民医院内分泌科 [3]复旦大学附属上海市第五人民医院高压氧室 [4]复旦大学附属上海市第五人民医院病理科,上海200240 [5]复旦大学上海医学院电镜室,上海200032

出　　处：《复旦学报（医学版）》2005年第1期43-46,共4页Fudan University Journal of Medical Sciences

摘　　要：目的 研究高压氧治疗(HBOT)是否可改善STZ(链脲佐菌素)大鼠的糖尿病性心肌病变。方法 STZ大 鼠造模成功3月,电镜证实有心肌病变后,随机分为3组:(1)HBOT组(n=30):每天接受0.15 MPa、HBOT 1 h,连 续20 d。(2)糖尿病对照组(n=32):不做治疗。(3)正常对照组(n=20):腹腔注射生理盐水的无糖尿病的正常大 鼠。分别在疗程5、10、20 d后处死治疗组及同期对照组大鼠,取心脏进行HE染色病理学观察和电镜观察。结果 HBOT治疗20 d后STZ大鼠的糖尿病性心肌病变明显好于同期糖尿病对照组,电镜观察显示肌丝排列整齐、Z线 明显、线粒体排列规则、结构紧凑。结论 HBOT可改善STZ大鼠糖尿病性心肌病变。Purpose To investigate whether hyperbaric oxygen therapy(HBOT) could improve diabetic cardiomyopathy in STZ-induced diabetic rats. Methods Diabetes was induced in 62 SD rats by single intraperitoneal injection of 1.0% STZ. Diabetic cardiomyopathy was showed by electron micrographs. The HBOT group (n=30) was treated with HBO (0.15 MPa for 1 h) for 20 days and insulin. The diabetic control group (n=32) was treated by only insulin. The normal control group (n=20) was not undertaken any treatment . The Morphological analyses was carried out by electron microscopy and expression of TGFβ1 by DAB method. Results Poorly organized cardiocytes with edema and vascular degeneration were observed before HBOT. Well organized cardiocytes without edema were found in HBOT group. Electron micrographs of ultrastructural observations on the cardiomyocytes showed well organized myofibrils with clear Z lines and normal mitochondria at day 20.Conclusions HBOT can improve diabetic cardiomyopathy in STZ-induced diabetic rats.

关 键 词：大鼠 糖尿病性心肌病 STZ 对照组 治疗作用 链脲佐菌素 高压氧 无糖 线粒体 电镜观察 

分 类 号：R587.1[医药卫生—内分泌] R542.2[医药卫生—内科学]