黑曲霉木聚糖酶在工业酒精酵母中的分泌表达  

Integrative expression of XynB of Aspergillus niger UV-11 in industrial yeast

作　　者：李海燕[1] 祝令香[1] 毛爱军[1] 董志扬[1] 

机构地区：[1]中国科学院微生物研究所微生物资源前期开发国家重点实验室,北京100080

出　　处：《微生物学报》2005年第1期135-138,共4页Acta Microbiologica Sinica

基　　金：国家"8 63计划"( 2 0 0 1AA2 14 15 1);国家自然科学基金面上项目 ( 3 0 170 2 18)~~

摘　　要：用重叠延伸PCR方法从黑曲霉 (Aspergillusniger)UV 11的基因组DNA中克隆出木聚糖酶的cDNA基因 ,构建了由酵母乙醇脱氢酶 (ADH1)启动子和终止子引导表达、木聚糖酶自身信号肽引导分泌、rDNA序列介导的酵母整合型分泌表达质粒pAX2。用pAX2与酵母YEp型G4 18抗性质粒共转化野生型工业酒精酵母S .cerevisiae 2 346 ,获得了整合型分泌表达木聚糖酶的酵母重组菌株XY2。The cDNA sequence of β-xylanase gene (xynB) was cloned from Aspergillus niger UV-11. It was inserted into the yeast expression vector and the recombinant plasmid pAX2 was obtained. The plasmid pAX2 was introduced into an industrial S. cerevisiae 2.346 and integrated into yeast genome by co-transformation of a YEPtype plasmid pBEJ16 carrying G418 resistance. The stable engineered yeast strain XY2 was obtained. It could express and secret extracellular xylanase, and enhance the alcohol production in wheat flour fermentation compared with the host strain S. cerevisiae 2.346.

关 键 词：木聚糖酶 整合分泌表达 酒精酵母工程菌 酒精生产率 

分 类 号：TQ223.1[化学工程—有机化工]