云南水牛的同期发情、超数排卵和胚胎移植试验  

Experiments on Synchronous Estrus, Superovulation and Embryo Transfer in Buffaloes of Yunnan

作　　者：和占星 [1] 和协超 [2] 罗在仁  朱芳贤 [1] 铃木达行  创向辉  尹安强  黄晓松  

机构地区：[1]云南省肉牛和牧草研究中心,云南,昆明,650212 [2]中国科学院昆明动物研究所,云南,昆明,650223 [3]盈江县畜牧局,云南,盈江,679300 [4]山口大学,日本,山口,753-8515 [5]德宏州畜牧兽医站,云南,潞西,678400

出　　处：《Zoological Research》2005年第1期106-111,共6页动物学研究（英文）

基　　金：云南省生殖生物学重点实验室开放基金 ;国家外专局智力引进项目

摘　　要：为探讨水牛胚胎移植的效果 ,于 2 0 0 2年对云南水牛进行了胚胎移植试验 :①用国产氯前列烯醇(PG) 0 6mg/头·次处理供、受体水牛的同期发情率和可用率分别为 4 3 33% (13/ 30 )和 16 6 7% (5 / 30 ) ;同期发情率经产水牛高于青年水牛 (P =0 0 86 ) ,杂交水牛高于德宏水牛 (P =0 15 3) ,体重 4 0 1～ 5 30kg水牛显著高于体重 30 0～ 4 0 0kg水牛 (P <0 0 5 ) ;发情明显水牛的可用率极显著高于发情不明显的水牛 (P <0 0 1)。②选用河流型摩拉水牛与沼泽型德宏水牛的杂交一代 5头为供体 ,分进口激素组 (n =2 )和国产激素组 (n =3)进行超数排卵 ,共有 2头获 9枚胚胎 ;进口激素组供体的平均获胚数和可移植胚数分别为 2 0枚和 1 5枚 ,比国产激素组分别多 0 33枚 (P =0 4 5 4 )和 1 17枚 (P =0 2 88)。③所获 4枚可用鲜胚分别移植 3头受体 ,结果 90d的妊娠率为 33 33% ,但最终无一头产犊。试验结果表明使用进口FSH 2 4mg +PG (Lutalyse○R) 35mg和国产FSH 11mg +PG 0 8mg对水牛超数排卵有效 ,同时提示需要足够数量的受体 ,从中选用发情表现明显、黄体发育良好的进行胚胎移植 ,才能取得良好效果。To investigate the effect of embryo transfer in buffaloes, buffaloes of Yunnan were experimented in 2002. Results showed: ①Synchronous estrus and usable rates of donors and recipients administered with cloprostenol (PG) 0.6 mg/head, injection once, were 43.33% (13/30) and 16.67% (5/30). Especially, the synchronous estrus rate was higher in parity's than in heifer's (P=0.086), higher in crossbred's than in Dehong Buffalo's (P=0.153), and higher in weight from 401 to 530 kg of buffalo cows than from 300 to 400 kg (P<0.05); And the usable rate of buffaloes with evidence estrus behavior was also higher than that of buffaloes with lack of evidence estrus (P<0.01). ②Five donors crossbred F 1 (Murrah×Dehong buffalo) were picked out from buffaloes of synchronous estrus and divided into two groups, i.e. import hormone group (n=2) and native hormone group (n=3), which were dealt with superovulation. The results showed that 2 out of 5 buffaloes gave 9 embryos, and average no. of recovered embryos and no. of transferable embryos in the import hormone were 2.0 and 1.5, and higher than those in the native hormone, which were 1.67 (P=0.454) and 0.33 (P=0.288), respectively. ③Four fresh embryos were transferred into 3 receipts, thereinto the rate of 90-day conception was 33.33%, but finally none gave birth to a calf. The experiment results indicate that it is in effect for superovulation of buffaloes with administered 24 mg of import FSH and plus PG (Lutalyse ○R ) 35 mg and the same as 11 mg of native FSH and plus PG 0.8 mg, meanwhile suggest that it is practicable if the receipt buffaloes are enough for synchronous estrus, then the cows with evidence estrus behavior and good CL checked can be selected for transferring embryos.

关 键 词：水牛 同期发情 超数排卵 胚胎移植 云南 氯前列烯醇 

分 类 号：S823.83[农业科学—畜牧学] S823.36[农业科学—畜牧兽医]