40%四氯化碳大鼠慢性肝损伤模型的研究  

A STUDY OF CHRONIC INJURY MODEL INDUCED BY 40% CARBON TETRACHLORIDE

作　　者：杨开选[1] 程薇波[2] 王文冬[2] 沈圣[3] 

机构地区：[1]四川大学华西附二院病理科,成都610041 [2]四川大学华西公共卫生学院病理教研室,610041 [3]四川大学华西公共卫生学院医检教研室,610041

出　　处：《现代预防医学》2005年第1期20-21,40,共3页Modern Preventive Medicine

摘　　要：目的 :研究 4 0 %四氯化碳致大鼠慢性肝损伤病理变化过程 ,完善 4 0 %四氯化碳慢性肝损伤的动物模型。方法 :雄性Wistar大鼠 80只 ,随机分为对照组和实验组。对照组每周两次给予溶剂玉米油 ,实验组每周两次给予 4 0 %四氯化碳 ,0 1ml/ 10 0g bw。于实验第 1、 3、 6、 8周染毒 4 8h后将动物处死 ,取肝组织作常规HE、Masson和Retculin染色 ,光镜下观察病理变化。结果 :染毒 1周后 ,中央静脉周围出现较多坏死肝细胞 ,并伴有气球样变和脂肪变性。染毒 3周后 ,气球样变和坏死进一步增多 ,并形成桥接坏死 ,局部网状纤维支架塌陷。染毒 6周后出现肝纤维化。染毒第 8周出现肝硬化。结论 :4 0 %四氯化碳使肝组织严重坏死 ,从而致肝组织第八周就出现明确的肝硬化。Objective:To research the pathological changes of chronic liver injury induced by carbon tetrachloride in rat.Methods:Eighty Wistar rats were divided into two groups including one control group and one experiment group at random . Experiment group was injected 40% carbon tetrachloride twice a week, 0.1ml/100g.bw .They were sacrificed at 48 hours after poisoning in the first, third, sixth and eighth week respectively, then the liver were taken out and sections stained by hematoxylin-eosin , Masson , Relculin were examined under light microscope.Results:Necrosis ,fatty degeneration and ballooning degeneration around centric veins were observed after poisoning in the 1st week. In the 3rd week , bridging necrosis were observed .And hepatic fibrosis and hepatocirrhosis appeared in the 6th and 8th week respectively.Conclusion: After poisoning with 40% carbon tetrachloride , the acute and chronic liver injuries get severer while the experiment undergoes ,hepatocirrhosis were observed in 8th week indeed.

关 键 词：40%四氯化碳 慢性肝损伤 病理变化 大鼠 给药方法 

分 类 号：R575[医药卫生—消化系统]