Nogo-A在成年大鼠脊髓和背根节的分布  

Immunohistochemical localization of Nogo-A in spinal cord and dorsal root ganglion of adult rat

作　　者：程希平[1] 刘惠玲[1] 宋朝君[2] 金伯泉[2] 焦西英[1] 游思维[1] 鞠躬[1] 

机构地区：[1]第四军医大学基础部全军神经科学研究所,陕西西安710032 [2]第四军医大学基础部免疫学教研室,陕西西安710032

出　　处：《神经科学通报》2005年第1期77-81,共5页Neuroscience Bulletin

基　　金：国家自然科学基金(30100195)

摘　　要：目的研究NogoA在成年正常大鼠脊髓和背根节的分布。方法免疫组织化学方法(ABC法)和免疫荧光双标记法。结果正常成年大鼠的脊髓灰质分布有大量的NogoA免疫阳性的寡突胶质细胞、运动神经元和中间神经元,免疫阳性反应产物主要分布于细胞的胞体和部分突起中。NogoA广泛分布于穿行于脊髓白质的纤维包裹的髓鞘和轴突上。在脊髓前根、后根和坐骨神经的运动和感觉的有髓和无髓纤维也可观察到NogoA的表达。而背根神经节的神经元也大量表达NogoA,其强度由弱至强不等,广泛分布于大、中、小各类感觉神经元的胞质及突起中。结论NogoA在成年大鼠脊髓,背根神经节和外周神经纤维的广泛存在提示其在正常状态下的神经功能中可能起重要作用。Objective To study the immunohistochemical localization of Nogo-A in the spinal cord and dorsal root ganglion of normal adult rat. Methods Immunohistochemical staining techniques (ABC methods) and double staining immunofluorescence techniques were used. Results There is a widespread expression of Nogo-A in the oligodendrocyte, motor neurons and inner neurons in the grey matter of the spinal cord of adult rat. Positive structures mainly located in the cytoplasm and some processes. Nogo-A was highly expressed in the axons and the myelin sheath surrounding fibers which pass through the white matter of spinal cord. Also Nogo-A immunoreactivity was intense in myelinated and unmyelinated axons in the ventral root, dorsal root and sciatic nerve. Similarly, most of dorsal root ganglion neurons expressed Nogo-A, with different immunoreactivity levels. The positive structures present in the cytoplasm and neurites of large, medium and small sense neurons. Conclusion The widespread expression of Nogo-A in spinal cord, dorsal root ganglion and peripheral nerves indicates that it possibly plays an important role on the functional activity of the nerve system in the normal situation.

关 键 词：Nogo—A 脊髓 背根神经节 外周神经 髓鞘 神经元 寡突胶质细胞 

分 类 号：R741[医药卫生—神经病学与精神病学]