人脂联素cDNA的克隆及序列分析  

Cloning and Sequencing of cDNA of Human Adiponectin

作　　者：刘德敏[1] 靳立忠[1] 孙颖[1] 张捷[1] 

机构地区：[1]天津医科大学代谢病医院,300070

出　　处：《天津医药》2005年第2期71-73,共3页Tianjin Medical Journal

基　　金：天津市教委资金资助项目(项目编号:20030325)

摘　　要：目的:构建人脂联素cDNA的克隆并进行序列分析。方法:提取人脂肪组织总RNA,运用RT-PCR方法扩增人脂联素cDNA完全编码区,将扩增产物通过TA克隆技术克隆至pMD18-T载体,对重组质粒进行测序验证。结果:成功构建人脂联素cDNA的克隆,重组质粒(pMD18-T/ADPN)经测序与Genebank检索的脂联素cDNA序列进行对比证实为100%同源。结论:所构建的人脂联素cDNA克隆适于进一步的克隆表达。Objective: To clone cDNA of human adiponectin for nucleotide sequencing. Methods: The total RNA of human adipose tissue was extracted.The cDNA complete coding region of human adiponectin was amplified by RT-PCR.The product was cloned into plasmid pMD18-Tby TA-clone.The recombinant plasmid was verified by sequencing. Results: The clone of cDNA of human adiponectin was successfully constructed.The recombinant plasmid(pMD18-T/ADPN)was verified to share 100%homologies with that of genebank by sequencing. Conclusion: The cloning of human adiponectin cDNA is adapted to further expression.

关 键 词：脂联素 CDNA序列 克隆表达 重组质粒 序列分析 测序 脂肪组织 T载体 克隆技术 CDNA克隆 

分 类 号：R587.1[医药卫生—内分泌] R589[医药卫生—内科学]