脑缺血及再灌流早期大鼠海马CA_1区NMDA受体的变化  

THE CHANGE OF NMDA RECEPTOR IN RAT HIPPOCAMPAL CA_1 AREA FOLLOWING TRANSIENT CEREBRAL ISCHEMIA

作　　者：冯志博[1] 陈子琏[2] 李峰[2] 

机构地区：[1]新乡医学院人体解剖学教研室,新乡453003 [2]中山大学医学院人体解剖学教研室,广州510080

出　　处：《神经解剖学杂志》2005年第1期73-76,共4页Chinese Journal of Neuroanatomy

摘　　要：探讨脑缺血和缺血再灌流早期海马CA1区NMDA受体的变化规律,选用雄性SD大鼠48只,随机分为:假手术对照组、单纯脑缺血15 min、20 min和30 min组及脑缺血15 min再灌流0 min、30 min和24 h组,参照Pulsinelli-Brierley(4VO)法制作脑缺血模型,取脑,连续冰冻冠状切片,NR1免疫组化染色并进行图像分析及计算阳性单位PU值.结果显示:(1)单纯脑缺血15min、20 min和30 min组PU值分别为5.89±0.92、5.18±1.63和4.89±2.69,与对照组PU值7.56±2.35相比,下降22.09%～35.32%(P≤0.05);(2)再灌流30min和24 h组PU值分别为4.20±1.37和2.99±1.28,与对照组PU值相比,减少44.44%～60.45%,有统计学意义(P＜0.05),均明显降低;(3)再灌流0 min、30 min和24 h组两两比较,24 h和0 min组的NMDA受体减少有统计学意义(P＜0.05).上述结果提示,脑缺血和缺血再灌流早期海马CA1区NMDA受体存在下行调节,这可能是脑缺血后自身的一种保护性调节.The present study was to study the change of NMDA receptors during the early phase of cerebral ischemia and ischemic reperfusion. 48 male SD rats were randomly divided into 6 groups: sham-operated control group, cerebral ischemia 15 min, 20 min, 30 min and cerebral ischemia 15 min reperfusion 0 min, 30 min, 24 h groups. The four-vessel occlusion(4-VO)ischemic model was performed with the method of Pulsinlli-Brierley in this experiment. Brain sections were obtained by cryostat sectioning, the section were prepared for NR1 immunohistochemistry staining and was observed by optical microscope. Quantitative analysis was carried out for NR1 immunoreactive spieces and PU value was calculated. The results showed that: (1) the PU values of hippocampal CA_1 area in the groups of cerebral ischemia(15 min, 20 min and 30 min)were 5.89±0.92, 5.18±1.63 and 4.89±2.69 respectively and the NR1 immunoreactive protein levels tended to decrease in contrast to the PU value (7.56±2.35) of the control group (decreased by 22.09%-35.32%)(P<0.05); (2) the PU values of hippcampal CA_1 area in the groups of reperfusion 30 min and 24 h were 4.20±1.37 and 2.99±1.28 respectively, and the NR1 immunoreactive protein levels significantly decreased by 44.44%-60.45% in contrast to that in the control group (P<0.05); (3) the PU values were compared between reperfusion 0 min, 30 min and 24 h groups. The reperfusion 24 h group was significantly lower than reperfusion 0 min group (P<0.05). The present results demonstrate that the decrease of NMDA receptors might be one of the mechanisms for protection against ischemia in the early phase of cerebral ischemia and ischemic reperfusion.

关 键 词：脑缺血 缺血再灌流 NMDA受体 对照组 早期 PU 大鼠海马 雄性 CA1区 SD大鼠 

分 类 号：R361[医药卫生—病理学]