聚合酶链反应预测宫颈癌的研究  

The prospect study of cervical carcinoma by PCR

作　　者：陈诵芬[1] 杨道理[1] 克丙申[1] 王宝成[1] 单乐媛[1] 谢华[1] 

机构地区：[1]山东济南军区总医院

出　　处：《中国优生与遗传杂志》1994年第3期9-11,共3页Chinese Journal of Birth Health & Heredity

摘　　要：采用聚合酶链反应对宫颈癌、宫颈糜烂、宫颈正常组织各５０例进行人乳头瘤病毒感染率的检测，据结果提示人乳头瘤病毒感染与宫颈癌发生的密切相关性，对预测宫颈癌的发生进行了前瞻性的研究。指出（ＰＣＲ）聚合酶链反应免除了昂贵繁杂的分子杂交过程，具有极高的敏感性是监测人乳头瘤病毒感染的“高危人群”、预测宫颈癌发生的最佳技术。samples including 50 patients with cervical carcinoma, 50 cervical erosion and 50 normal cervical tissue were studied,The relationship between cervical carcinoma and HPV infection was studied by polymerase chain reaction(PCR).The PCR result showed that the HPV16 infection rate in cervical carcinoma statostically is more than that in cervical erosion and normal tissue (P<0.01),It suggests that the HPV16 may be the main cause to cervical carcinoma. HPV18 has same expression as HPV16 see that they showed different HPV16 infection rate in the tissue from around the cancer,erosion and normal cervical smear,The HPV infection may be the important factor to cause cancer. It suggests the patients with HPV infection are high-risk group and should be long-term followed up. The method to study the HPV infection in clinical by PCR has been proved more sensitive, economic and simple. It can not only test the HPV, and may be an effective method used in general investigation prospectively to disco ver the high-risk group to cervical cancer.Moreover,we can get plentiful material by PCR from the paraffin-embedded tissue for retrospective study.

关 键 词：宫颈癌 聚合酶链反应 人乳头瘤病毒感染 癌发生 正常组织 宫颈糜烂 高危人群 分子杂交 PCR) 

分 类 号：R737.33[医药卫生—肿瘤] R711[医药卫生—临床医学]