用核酸分子杂交技术产前诊断人巨细胞病毒感染  

Prenatal diagnosis of human cytomegalovirus infection by nucleic acid hybridization

作　　者：陶幼慈[1] 孙永玉[1] 蒋晓薇[1] 

机构地区：[1]同济医科大学附属协和医院妇产科遗传室

出　　处：《中国优生与遗传杂志》1995年第6期15-17,共3页Chinese Journal of Birth Health & Heredity

摘　　要：用ＥＬＩＳＡ法筛选早孕妇女和中孕引产妇女血人巨细胞病毒ＩｇＭ（ＨＣＭＶ－ＩｇＭ），早孕妇女阳性者在孕１２－２０周（中孕引产妇女阳性者在引产时）留羊水，用生物素标记的ＨＣＭＶ－ＤＮＡ探针斑点杂支检测其羊水中的ＨＣＭＶ－ＤＮＡ，对胎儿巨细胞病毒感染作出产前诊断。结果：早孕妇女３８４例，血ＨＣＭＶ－ＩｇＭ阳性２６例。此２６例羊水ＨＣＭＶ－ＤＮＡ阳性９例，其中７例出生时留脐血进一步检测ＨＣＭＶ－ＩｇＭ和ＨＣＭＶ－ＤＮＡ。证实有ＨＣＭＶ感染。中孕引产妇女１２５例，血ＨＣＭＶ－ＩｇＭ阳性１０例。此１０例羊水ＨＣＭＶ－ＤＮＡ阳性４例。Human cytomegalovirus IgM (HCMV-IgM)in blood of pregnant women in the first trimester and midgestation induction was detected by ELISA.For pregnant women of HCMV-IgM positive in 12-20 weeks'gestation,amniotic fluids(10ml)were collected and HCMV-DNA in amniotic fluid was detected by dot blot hybridization with biotinlabelled probe in order to diagnose fetal HCMV infection before birth. Results: of the 384 pregnant wonen in the first trimester,26(6.77%)were positive for HCMV-IgM in blood,9 cases out of the above 26 cases were positive for HCMV-DNA in amniotic fluids,7 out of 9 umbilical cord blood were collected at delivery for further examination of HCMV-IgM and HCMV-DNA.The results were consistent with prenatal examination.Of the 125 women who made induction in the second trimester, 10 were positive for HCMV-IgM in blood. Of 10,4 were positive for HCMV-DNA in amniotic fluids.

关 键 词：阳性 HCMV-DNA HCMV-IGM 中孕引产 早孕 产前诊断 妇女 核酸分子杂交 探针 斑点 

分 类 号：R714[医药卫生—妇产科学] R169.42[医药卫生—临床医学]