黄瓜幼苗发育及冷锻炼过程子叶中的多肽变化  

POLYPEPTIDE CHANGES IN COTYLEDONS DURING CUCUMBER SEEDLING DEVELOPMENT AND CHILLING ACCLIMATION

作　　者：李晓萍[1] 郭俊彦[1] 

机构地区：[1]中国科学院华南植物研究所

出　　处：《Acta Botanica Sinica》1993年第10期766-771,共6页Acta Botanica Sinica（植物学报：英文版）

基　　金：研究所所长青年科学基金资助课题

摘　　要：黄瓜幼苗在发育过程中抗冷性逐渐降低，光下冷锻炼可以提高幼苗的抗冷性，如果锻炼中不给予光照，则幼苗的抗冷性降低。用双向电泳结合银染的方法检测表明：幼苗在６－１２日龄的发育过程中，一些多肽消失，一些则形成。光，暗锻炼对消失的多肽的延缓和对新形成的多肽的促进效果不同。光，暗锻炼均诱导了一个５８．０ｋＤ多肽的形成。认为在冷锻炼中有多个基因参与调控，锻炼所诱导的抗冷性的提高是基于与发育有关的代谢的协调。The changes of chilling resistance and polypeptide pattern during cucumber (Cucu-mis sativus L. ) seedling development and during chilling acclimation in light (CAL) and chilling acclimation in dark (CAD) have been studied. The results showed that the chilling resistance decreased gradually during seedling development,CAL could enhance chilling resistance but CAD made the seedlings more sensitive to chilling temperature Two dimension PAGE study showed that during 6-12 days old seedling development some polypeptides disappeared and some appeared . CAL and CAD had different effects on those polypeptides. Both CAL and CAD induced a 58. 0 kD polypeptide. There might be cooperation among several genes during chilling acclimation. Chilling endurance obtained from acclimation is based on the integration of metabolism correlated with development.

关 键 词：冷锻炼 多肽 图谱 黄瓜 苗 发育 

分 类 号：S642.201[农业科学—蔬菜学]