应用Ach—ISMs检测在体大白鼠脑梗塞区Ach变化  

Detecting changes of Ach in infarction areas in rats in vivo with Ach--TSMs

作　　者：耿东进[1] 赵瑜[1] 成柏华[2] 

机构地区：[1]上海第二医科大学附属仁济医院神经科 [2]上海第二医科大学传统医学研究中心针灸室

出　　处：《中风与神经疾病杂志》1993年第1期8-10,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：我们在合成了对乙酰胆碱敏感的活性材料的基础上进一步制备了乙酰胆碱微电极(Ach—ISMs)。本实验用雄性大白鼠10只自身对照,检测正常脑皮层及大脑中动脉凝闭(MCAO)后不同时间缺血区皮层细胞外Ach浓度的变化。结果显示正常脑皮层细胞外Ach浓度为0.71±0.40mM((?)±SD).MCAO后1—2分钟梗塞区Ach浓度迅速升高到1.98±0.62mM((?)±SD).随着梗塞时间延长Ach浓度有逐步恢复到正常的趋势。本实验还对Ach—ISMs的性能作了测试。We had synthesized sensor for Ach and made Ach—ISMs. This paper reported changes of Ach concentration in normal cerebral cortex and in infarct areas after MCAO at different time in 10 male Wistar rats. The results showed: the concentration of Ach in normal cerebral cortex were 0.71±0.40raM (X±SD),the concentration of Achin infarct areas rose rapidly to 1.98±0.62mM (X±SD)after1—2 minutes of MACO,the concentration of Ach had a direction to recover to normal with long time after MCAO. This paper also discussed the function of Ach——ISMs.

关 键 词：脑栓塞 乙酰胆碱 动脉凝闭 

分 类 号：R743.330.2[医药卫生—神经病学与精神病学]