测定胃肠道恶性肿瘤和肺癌组织中u-PA的意义  

Significance of measuring urokinase type plasminogen activator in carcinoma tissues of the gastroinstestinal and lung malignant tumors

作　　者：张啓良 陈岚[1] 倪玉英[1] 韩欣[1] 徐也鲁[1] 王振义[1] 

机构地区：[1]上海第二医科大学病理生理教研室上海血液学研究所

出　　处：《中国病理生理杂志》1993年第1期36-39,共4页Chinese Journal of Pathophysiology

摘　　要：本文建立了测定微量尿激酶型纤溶酶原激活物(u-PA)抗原的ELISA法,检测35例胃肠道恶性肿瘤如胃、食道、结肠、直肠癌以及肺癌患者的癌组织与癌邻近组织的u-PA含量,结果各种癌组织的u-PA明显增高(P<0.05～0.01)。同时测定组织纤溶酶原激活物(t-PA)抗原,仅胃癌组织有明显增高(P<0.01),纤溶酶原激活物(PA)的活性增高见于胃、结肠和直肠癌组织(P<0.05～0.001)。提示这些恶性肿瘤细胞产生或分泌PA抗原特别是u-PA增多,使局部纤溶活性增强,是促进癌细胞增殖和转移的机制之一。The urokinase type plasminogen activator (u-PA) antigen level in tumorand adjacent tissues was evaluated in 35 cases of gastrointestinal carcinomas and lungcancer with the u-PA ELISA sandwich method developed in our laboratory. The concentrations of u-PA antigen in 5. 0ml of extracts, each of them made from0. 5 gram tumor tissue, were 118. 4±121. 1, 138. 8±115. 8, 103. 3±56. 7, 85. 8±54. 5 and197. 4±86. 3 μg/L for the cases of gastric (n=10), esophagus (n=8), colon (n=6),rectum (n=6) carcinoma and lung cancer (n=5), respectively. It showed that the u-PAantigen levels in all the 5 kinds of tumor tissue were significantly higher than that oftheir adjacent tissues (P<0. 05～0. 01). On the other hand, the same samples were alsoused to analyse tissue type plasminogen activator (t-PA) antigen and the activity ofplasminogen activators (PAs). Significantly elevated levels were found for PAs activityin tumor tissues in the cases of gastric. colon and rectum carcinoma. and for t-PA anti-gen, higher level was only found in the gastric tumor. These results may be explained as that the elevation of u-PA antigen level was oneof the features of some kinds of tumor tissues and contributed to cell growth and/ormetastasis for the malignant tumors.

关 键 词：纤溶酶原 激活物 肿瘤转移 抗体 

分 类 号：R730.2[医药卫生—肿瘤]