脑缺血时海马细胞外液中兴奋性氨基酸的变化  

The changes in extracellular excitatory amino acid in rat hippocampus following cerebral ischemia

作　　者：李宁[1] 吴建中[1] 蒋京生 

机构地区：[1]北京市神经外科研究所,北京100050

出　　处：《中国病理生理杂志》1993年第6期740-744,共5页Chinese Journal of Pathophysiology

摘　　要：近几年脑内微量透析技术在国外已广泛应用于研究脑细胞间隙液中神经递质的变化,国内尚未见这方面的报道。本文用此技术,观察大鼠在不同程度缺血情况下,海马细胞外液中兴奋性氨基酸(EAA)(谷氨酸Glu,天门冬氨酸ASP)的变化。用0.3mm直径的透析管横穿大鼠双侧海马,随后用人工脑脊液以1μl/min的速度灌注。脑缺血模型用电解式氢清除法测定脑血流的变化。短暂性不完全性脑缺血,Glu和Asp分别升高到8.9±4.8,1.09±0.4μmol。短暂性完全性脑缺血,Glu、Asp分别升高到18.4±13.?,3.9±3.1μmol。这些变化随血流恢复而复原。持续性完全性脑缺血Glu、Asp分别升高到61.7±16.8,13.4±9.0μmol。相关系数r=0.646,证实脑缺血程度与EAA释放呈正相关。Intracerebral microdialysis was used extensively for the study of thechanges of neurotransmitters in brain interstitial space. Extracellular glutamate(Glu), aspar-tate(Asp) in rat hippocampus with difforent cerebral ischemia were measuered by usingmicrodialysis. Rats were implanted with 0.3 mm diameter dialysis tubing through thehippocampus and subsequently perfused with artificial cerebrospinal fluid at a flow rate of 1μl/min. Three types of cerebral ischemia were produced by a combination of carotidartery (CA) clamping and hypotension. Cerebral blood flow was measured by hydrogen clea-rance in electrolysis. In the transient incomplete cerebral ischemia (CA clamping 10 min).The Glu and Asp contents of extracellular fluid were increased 2.7 and 2.3 flods respec-tively. In the transient complete ischemia (CA clamping and hypotension 10 min) Gluand Asp increased 4.8 and 5.0 flods. These increases were revivable while cerebral bloodflow was recovered. Complete ischemia in long duration, Glu and Asp increased 17.5 and15 flods respectively. There were definite relation between the changes of extracellularexcitatory amino acid content and the difference in cerebral ischemia.

关 键 词：脑缺血 谷氨酸盐类 天冬氨酸 海马 

分 类 号：R743.31[医药卫生—神经病学与精神病学]