RT-PCR扩增Ⅰ型登革病毒核酸及其临床应用  

Amplification of Dengue Virus Type 1 RNA by RTPCR and Its Clinical Application

作　　者：李刚[1] 郭日波[1] 王飞[1] 瘳育煌 

机构地区：[1]中山医科大学传染病学教研室,广州510630 [2]广州市卫生防疫站

出　　处：《中国公共卫生学报》1993年第4期193-195,共3页

摘　　要：应用逆转录聚合酶链反应(RT-PCR)技术扩增I型登革病毒E基因部分片段。所设计的引物1位于碱基序列的第1537～1557,引物2位于1318～1336,扩增产物240bp,对I型登革病毒特异。通过检测证明RT-PCR可测出少至5TCID_(50)的病毒RNA。经对11份病毒分离与免疫荧光分型证实为I型登革热病人的血清和9份疑似I型登革热但病毒分离阴性的急性期病人血清检测,证明RT-PCR敏感性明显高于病毒分离的敏感性。可用于登革热的早期快速诊断。Reverse transcription-polymerase chain reaction (RT- PCR) was developed for the amplification of partial E genome of dengue virus type 1 (D-1). Two primers specific for D-1 bracketed a 240-nucleotide sequence, corresponding to bases 1318~1557 in the D-1 genomic RNA sequence. RT-PCR can detect dengue viral RNA from at least 5TCID_(50) virus, whch was justified by detection of serial dilutions of culture supernatants. Moreover. we have also applied RT-PCR to 20 serum samples in which 11 were found positive by culture and 9 negative. Our findings showed that RT-PCR was more rapid and sensitive than cell culture in diagnosis of dengue fever.

关 键 词：登革热病毒 聚合酶链反应 

分 类 号：R373.33[医药卫生—病原生物学]