反相离子对高效液相色谱法测定谷胱甘肽滴眼液含量  

DETERMINATION OF GLUTATHIONE EYE DRROPS BY REVERSED PHASE ION-PAIR HPLC

作　　者：郭福庆[1] 

机构地区：[1]天津市药品检验所,300070

出　　处：《中国药品标准》2000年第3期35-38,共4页Drug Standards of China

摘　　要：目的:建立谷胱甘肽滴眼液的HPLC含量测定方法。方法:色谱条件为:C_(18)柱(150mm×4.6mm,10μm),流动相:0.01 mol·L^(-1)磷酸二氢钾溶液—乙腈(85:15)(其中含戊烷磺酸钠0.01mmol·L^(-1),用磷酸调pH值至2.5,间苯二酚为内标物,检测波长:210nm,流速:0.8ml·min^(-1),测定温度:35℃。结果:谷胱甘肽浓度在10～300mg·L^(-1)之间时,其与内标物的浓度比和其相应的峰面积比呈良好的线性关系,回归方程为:Y=7.0197X—8.3200×10^(-3),r=0.9999。对照品的峰面积与内标物的峰面积比的相对标准偏差为0.86％(n=6)。被测溶液在24h内与内标物的峰面积之比值基本不变。结论:本方法简便,灵敏,准确,可靠。Objective: A RP Ion—pair HPLC method for determination of glutathione eye drops was developed. Methods: The HPLC method for determination of glutathione was: C_(18) column (150mm×4.6ram, 10μm), mobile phase. 0.01 mol/L potassium dihydrogen phosphate—acetonitrile (containning 0.01 mmol/L sodium pentylsulfonate; adjust pH value to 2.5 with phosphoric acid)(85:15), detector wavelength: 210nm, flow rate: 0.8ml/min, resorcinol was internal standard. Results: The linear relationship of calibration curves was good in the range of 10～300mg/L(r=0.9999). The average recovery and RSD of glutathione was 99.9%, 0.86%. Conclusion:The method was simple rapid and erliable

关 键 词：滴眼液 谷胱甘肽 内标物 高效液相色谱法 反相离子 含量 对照品 磺酸 间苯二酚 戊烷 

分 类 号：R927.2[医药卫生—药学] R284