血清骨唾液酸蛋白的生物变异性研究  

Biological variability of serum immunoreactive bone sialoprotein

作　　者：李裕明[1] HW．Woitge R．Ziegler MJ．Seibel 

机构地区：[1]同济医科大学附属协和医院内分泌科,武汉430022 [2]德国海德堡大学医学院内科I医院

出　　处：《中国骨质疏松杂志》1999年第4期4-7,共4页Chinese Journal of Osteoporosis

摘　　要：目的　骨唾液酸蛋白（Ｂｏｎｅ ｓｉａｌｏｐｒｏｔｅｉｎ，简称ＢＳＰ）是由成骨细胞分泌的一种非胶原蛋白质，反映骨转换和骨形成的指标。最近研究认为：血清ＢＳＰ浓度可反映破骨细胞活性和骨吸收过程，也可能是一个骨吸收指标。本实验测定了血清ＢＳＰ在人体内的生物变异性。方法　采用ＲＩＡ 法测定了２９０例不同性别和年龄的正常人血清ＢＳＰ的正常值，血清ＢＳＰ的天－天变异性及２４ ｈ 生物周期。　结果　在儿童组血清ＢＳＰ正常水平明显高于成人组，其最高值在新生儿期和青春期。绝经后妇女其血清浓度比绝经前妇女水平明显升高（Ｐ＜ ０．０５）。血清ＢＳＰ在每天同一时间的波动范围在 ７．３％ 至１７．７％ （平均１１．７％ ）。２４ ｈ 内有一个明显的生物周期性变化，表现为峰值在凌晨４～８ 时，然后逐渐下降直到午后１４时为最低。其生物周期的最大波幅为±２０％ ，（平均血浓度为１０．５ ｎｇ／ｍ ｌ）。结论　血清ＢＳＰ反映了骨转换的生理变化与年龄有明显相关性，其血清水平２４ ｈ 内有一个明显的生物周期，而天与天之间变异性较小。Objective\ Serum immunoreactive bone sialoprotein(sBSP) accounts for approximately 10% of the non collagenous proteins of bone.Recent findings suggest that its serum concentrations reflect processes linked to osteoclast activity and bone resorption. Methods In order to determine the biological variability of serum BSP,the following studies have been performed:i)measurements of serum BSP in different genders and age groups in healthy subjects(n=290);ii)day to day variation(n=9);and iii)diurnal variation(n=10).Serum BSP values were quantified using a newly developed polyclonal RIA for human BSP.Results Serum BSP levels were significantly higher in children and adolescents,with highest values during early childhood and during puberty.In postmenopausal women,serum BSP levels were significantly higher,compared with premenopausal women( P <0 05).Day to day variability ranged between 7 3 and 17 7%(mean:11 7%).Diurnal variation showed a peak between 04:00 and 08:00 a.m.,followed by a steady decline until noon.The maximum amplitude of this diurnal change was approximately ±20%(mean BSP concentraton 10 5 ng/ml).Conclusions Serum BSP concentrations indicate increased bone turnover soon after the menopause,reflect the pubertal growth spurt,show little day to day variability.and exhibit significant diurnal variation.

关 键 词：血清 唾液酸 骨转换 骨吸收 蛋白 异性 年龄 生物变异 不同性别 绝经前妇女 

分 类 号：R681[医药卫生—骨科学] R446[医药卫生—外科学]