新肽类抗生素B-3543的研究——Ⅱ.发酵、分离、纯化及理化性质(英文)  

STUDIES ON A NEW PEPTIDE ANTIBIOTIC B-3533 Ⅱ. FERMENTATION, ISOLATION, PURIFICATION AND PHYSICO-CHEMICAL PROPERTIES

作　　者：蒋振海 黄友亮 汪万青 杨自文 梁翠霞 谢万诚 小峰则男 田口龙佑 江口润 宫畸幸雄 

机构地区：[1]中国武汉,湖北省农科院植保所430064 [2]日本426亍静冈县藤枝市源助301科研制药株式会社中央研究所

出　　处：《中国抗生素杂志》1993年第2期124-127,135,共5页Chinese Journal of Antibiotics

摘　　要：采用棉籽饼粉培养基,发酵培养144小时,新抗生素B-3543的效价可达3000μg/ml,通过CAD-40柱, DEAE-Sephadex A-25柱和硅胶柱进行分离和纯化,可制得白色粉末状的B-3543纯品。根据该素的理化性质和氨基酸组成,将其鉴定为一种含有15种氨基酸的新肽类抗生素。The optimal medium for fermentation was pharmamedia medium. The antibiotic titer reached about 3,000μg/ml after 144 hours of fermentation. The white powder of antibiotic B-3543 was obtained after purification by column chroma-tography on CAD-40, DEAE-Sephadex A-25 and silica gel, according to the physico-chemical propsrties and constituent of amino acids of antibiotic B-3543, it is considered to be a new peptide antibiotic consisting of 15 kinds of amino acids.

关 键 词：发酵 分离 抗菌素 真菌B-3543 

分 类 号：TQ465.92[化学工程—制药化工]