黄参膏对胃癌癌前病变细胞凋亡及BCL-2蛋白表达的影响  

The effects of Huang shen ointment on apoptosis and expression of bcl-2 in gastric precancerous lesions

作　　者：陈惠新[1] 许岸高[1] 刘集鸿[1] 

机构地区：[1]广东惠州市中心人民医院消化内科

出　　处：《现代消化及介入诊疗》1999年第4期28-29,共2页Modern Interventional Diagnosis and Treatment in Gastroenterology

摘　　要：目的:探讨黄参膏对胃癌癌前病变细胞凋亡及相关基因表达的影响。方法:将46例胃癌癌前病变患者随机分为2组,治疗组28例,口服黄参膏60ml/天,对照组18例,口服维酶素片2.4g/天,疗程2月,治疗前后检测细胞凋亡指数和Bcl-2蛋白的表达。采用原位末端标记法(TUNEL)检测细胞凋亡,采用免疫组化检测Bcl—2蛋白的表达。结果:黄参膏组服药前后细胞凋亡指数分别为19.8±9.6％和32.8±16.2％,服药后细胞凋亡指数显著高于服药前(P<0.05)。Bcl-2蛋白阳性率治疗前后分别为53.6％和28.6％,治疗前后比较具有显著性差异(P<0.05)。而对照组细胞凋亡指数和Bel-2蛋白阳性率在服药前后比较均无显著性差异(P>0.05)。结论:黄参膏可诱导胃癌癌前病变细胞凋亡。To evaluate the effects of Huang shen ointment on apopfosis and expression of Bcl-2 in gastric precaucerous lesions. Methods Foirty-six cases of gastric precancerous lesions were divded into two groups at random—a treatment group of 28 cases and a control group of 15 cases. The treatment group was treated with Huang shen ointment 60ml a day for two months. The control group was treated with wei mei su 2.4g a day for two mouths. TUNEL method was used to detect apoptosis. Immunohistochmical analysis was used to detect the expression of bcl-2 protein. Results The treatment group changed as follow: Apoptosis indexes increased from 19.8±9.6％ to 32.8±16.2％, The expression rates of bcl-2 decreased from 53.6％ to 28.6％. The changes of apoptosis indexes and expression rates of bcl-2 were statistically significant (P<0.05). In 18 control cases it did not change significantly (P>0.05). Conclusions It is necessory to study the therapeutic value of Huang sheng ointment on gastric precancerouslesions, ince it can induce apoptosis of gastric precancerous lesions cells.

关 键 词：胃癌癌前病变 细胞调亡 BCL-2蛋白 治疗前后 细胞凋亡 BCL-2蛋白表达 对照组 口服 服药 阳性率 

分 类 号：R735.2[医药卫生—肿瘤] R273.52[医药卫生—临床医学]