醛固酮合成酶基因CYP11B2在肝储脂细胞中的表达  

The Expression of CYP11B2 in Fat Storing Cells of Liver

作　　者：李旭[1] 盂莹 杨希山[1] 吴平生[3] 

机构地区：[1]南方医院全军消化研究所,广州510515 [2]南方医院全军消化研究所 呼吸科,广州510515 [3]南方医院全军消化研究所 心内科,广州510515

出　　处：《现代消化及介入诊疗》1999年第4期35-36,共2页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：国家自然基金(39870331)

摘　　要：目的:检测醛固酮合成酶基因CYP11B2在肝储脂细胞中的表达。方法:异硫氰酸胍法提取总RNA,逆转录扩增CYP11B2。原位杂交检测肝组织CYP11B2的表达。结果:CYP11B2的表达定位于肝储脂细胞。结论:肝储脂细胞能表达CYP11B2。To investigate the possibiltiy that CYP11B2 is expressed in liver. Methods By means of reverse transcriptase polymerse chain reaction and in situ hybridization,the expressions of aldosterone synthase gene-CYP11B2 mRNA were detected. Results The expression of CYP11B2 mRNA is localized in the endoplasm of fat storing cells. Conclusion CYP11B2 is expressed in fat storing cells.

关 键 词：CYP11B2 脂细胞 表达 醛固酮合成酶 酶基因 原位杂交检测 肝组织 逆转录 扩增 RNA 

分 类 号：R544.1[医药卫生—心血管疾病] R589.2[医药卫生—内科学]