提取程度及电泳上样量对布氏菌外膜蛋白电泳图谱恒定性的影响  

The Effect of the Extracting Extent and the Loading during Electrophoresis on the Stability of the Pattern of SDS-PAGE in Brucella

作　　者：武素怀 姜淑贤[1] 马烨[1] 何丽华 尚德秋[1] 李兰玉[1] 

机构地区：[1]中国预防医学科学院流行病学微生物学研究所

出　　处：《中国人兽共患病杂志》1993年第2期21-22,共2页Chinese Journal of Zoonoses

摘　　要：由于难以从布氏菌的外膜蛋白中将干扰因素多糖成分去除到恒定水平的最低限度,迄今未能获得可用于分类的恒定的外膜蛋白电泳图谱。我们最大限度地提取出外膜蛋白及牢固附于其上的多糖成分;在电泳过程中又以超载的手段促使该二成分的相互作用达到综合平衡状态。由此可以消除不等程度多糖的干扰而产生的外膜蛋白电泳谱带的不恒定。为揭示了不同种布氏菌微孔蛋白热解聚前后电泳谱带变化规律及其所表现出的种的特异性奠定了基础。Because of adhering tightly between the molecular of outer membrane proteins and polysaccharides, so up tonow it could not be obtainedf33a stable pattern of SDS-PAGE of Omp, in Brucella- A new train of thought wasprovided. In this way, allowing the adhesion between Omp and poiysaccharides achieved at the largest degreef33complete equilibrium by extract using a dipolar ionic detergentf33Zwittergent 3-14 and by overloading sample inelectrophoresis. So that elimination of different degree of adhesion of Polysaccharides to the molecular of Omps.

关 键 词：外膜蛋白 布鲁氏杆菌 电泳图谱 

分 类 号：R378.5[医药卫生—病原生物学]