侧脑室注射腺苷对海马痛单位的影响及其可能机制  

EFFECT OF ADENOSINE INJECTED INTO THE LATERAL VENTRICLE ON PAIN UNITS OF HIPPOCAMPUS

作　　者：覃筱燕[1] 莫书荣[1] 

机构地区：[1]广西医科大学生理教研室

出　　处：《广西医科大学学报》2001年第2期179-181,共3页Journal of Guangxi Medical University

摘　　要：目的 :探讨腺苷 (Ado)对海马痛单位的影响及其可能机制。方法 :采用侧脑室给药法 ,参照 Sawyer兔脑图谱 ,用 SN-2型推进器以 2μm/ s速度将玻璃微电极插入海马 P2～ 5 ,L3～ 6 ,H6～ 9处引导神经元自发放电。在 P1 L4,5 H6 处埋藏套管 ,用牙托水配牙托粉固定 ,作脑室注射用。结果 :共引导 35 8个海马放电神经元 ,选择对伤害性刺激起反应的痛单位 80个 ,侧脑室分别注射不同剂量的腺苷及腺苷非选择性受体拮抗剂 8-苯茶碱 (8- PT)、腺苷 A1 受体选择性拮抗剂 8-环戊 - 1,3-二现代在嘌呤(DPCPX)和 ATP敏感性钾通道抑制剂格列苯脲 (Gli)后 ,发现腺苷能抑制大部分海马痛单位放电活 ,且呈明显的剂量依赖性。而腺苷的上述抑制作用均被 8- PT,DPCPX所阻断。格列苯脲能翻转腺苷对海马痛单位放电的抑制作用 (P <0 .0 0 1)。结论 :腺苷能抑制海马痛神经元的放电活动 ,其人造用机制可能与腺苷作用于海马痛神经元上的 A1 受体而引起神经元细胞膜上的Objective:This study investigates the effect of intracerebroventricular injection of Ado on the pain units of hippocampus (HPC)and probable mechanism in conscious paralytic rabbits Mothods:Referring to Sawyers “The rabbit diencephalons in stereotaxic coordix nates”,putting glass microelectrodes into P 2~5 L 3~6 H 6~9 of the HPC in 2 μm/s by SN 2 to induce discharge of neurons Results:The most discharged electric frequency of HPC decreased significantly after injecting Ado in different doses (50,100,200 μ mol/10 μl)into the lateral vertricle of rabbits after 15,30,50 min,and this effect is in a dose dependent manner Both Ado non selective receptor antagonist 8 PT (50 mmol/10 μl)and Ado selective A 1 receptor antagonist DPCPX (50 nmol/10μl)completely blocked the inhibitory effects of Ado Atp sensitive K + channel blocker Gli (70 mmol/10 μl) also abolished this inhibitive effect of Ado Conclusion:Ado could inhibit the spontaneous discharged electric frequency of HPC pain units in a dose dependent manner;Gli could abolish this inhibitive effect of Ado,suggesting that inhibitive effect of Ado on pain units of HPC is related to Ado binding with A 1 recepror on neurons,resulting in an activation of ATP sensitive K + channels

关 键 词：腺苷 格列苯脲 海马 痛单位 

分 类 号：R363[医药卫生—病理学]