狂犬病病毒快速荧光抗体技术测定的研究  

A rapid fluorescent antibody technique for monitoring rabies virus

作　　者：张砚锦 唐桂运[1] 邵振华[1] 

机构地区：[1]中国兽药监察所,北京100081

出　　处：《中国兽医科技》1993年第6期3-4,共2页Chinese Journal of Veterinary Science and Technology

摘　　要：本试验用自制兔抗狂犬病病毒荧光抗体,检测病毒在BHK_(21)-C_(13)单 层细胞中的增殖,建立了快速荧光抗体技术(RFAT)用于狂犬病病毒TCID_(50)的 测定。用RFAT和小白鼠脑内接种半数感染量(MID_(50))测定法对狂犬病病毒 ERA株毒液32批,冻干种毒两批和Flury-LEP株冻干苗11批进行了平行测定,并对测毒结果进行了统计学分析。病毒滴度平均值(log10):ERA株TCID_(50)/ml为6.16,MID_(50)/0.03ml为4.86;Flury-LEP株T CID_(50)/ml为5.85,MID_(50)/0.03ml为4.74。ERA株TCID_(50)和MID_(50)相关系数为0.826,Flury-LEP株为0.754。结果表明在可靠性和可重复性方面RFAT至少同MID_(50)测定法相同。但RFAT简便、快速,是替代MID_(50)测定法进行病毒测定的理想方法。Using free of serum monolayer cell culture, rabies virus was concentrated and purified as neutralization antigen. A neutralizing enzyme-linked immunosorbent assay (N-ELISA) was established on microtiter plate. 40 serum samples from sheep post-vaccinated with vaccine were tested by the N-ELISA. And the results were compared with those of mouse neutralization test (MNT). The mean antibody titer of N-ELISA was I:26.4 and that of MNT was I:25.5. The correlation coefficient of the two methods was 0.912. The method can replace the MNT for vaccine efficacy evaluation and seroepidemiological surveillance.

关 键 词：狂犬病病毒 测定 荧光抗体 

分 类 号：S852.655[农业科学—基础兽医学]