L1210细胞系克隆株的某些细胞学特性比较  

Comparison of Some Cytology Characteristics among L1210 Cell Lines

作　　者：刘秋燕[1] 吕占军[1] 王秀芳[1] 刘福英[1] 

机构地区：[1]河北医科大学实验动物学部,石家庄050017

出　　处：《中国实验动物学杂志》2001年第2期72-77,共6页Chinese Journal of Laboratory Animal Science

基　　金：国家"九五"攻关课题! (96 -A2 3 -0 6 -0 3)

摘　　要：目的　对L12 10细胞系进行细胞克隆 ,筛选出生长旺盛的细胞克隆 ,对不同克隆细胞及原代细胞的某些细胞学特性进行比较研究。方法　扫描电镜观察细胞表面结构 ;用流式细胞仪测定细胞DNA含量 ;免疫组化染色法测定细胞蛋白质的分布。结果　扫描电镜观察细胞表面结构 ,克隆株L2B9、L2H7、L2H8、L3B12及L3E11都有大量微嵴、微皱褶 ,但疏密程度不同 ;L2C9的微嵴和微皱褶较为粗大且有丰富的微绒毛 ;L3F9细胞表面有大量的突起。克隆细胞株DNA含量从小到大依次为L2H7、L3F9(8 3pg)、L2H8(8 5pg)、L2C9(8 6pg)、L3B12 (8 7pg)、L2B9(8 9pg)及L3E11(9 0pg) ;原代细胞DAN含量为 8 8pg。克隆细胞染色体数目分别为 48,5 8,6 4,6 6 ,6 2 ,6 3,5 8条 ,最少为 48条 (L2B9) ,最多为 6 6条 (L3B12 ) ;原代细胞染色体数目从 12～ 78条不等 ,主流染色体为 6 2～ 6 3条 ,所占比例为2 1 6 %。免疫组化染色结果显示 ,不同克隆细胞与 10种抗体反应阳性率及阳性反应程度不同 ;且克隆细胞反应阳性率普遍高于原代细胞。结论　不同克隆细胞之间某些细胞学特性有差别 ,说明原代L12 10细胞间已有明显异质性 ;克隆细胞较原代细胞的均一性好。Objective To isolate L1210 cell lines by the single cell cloning technique and to compare some cytology characteristics of L1210 cells and clonal cells.Methods By scanning electron microscope we observed cell superficial structure; DNA content analysis by flow cytometry; the protein distribution analysis by immunohistochemistry. Results Clonal cells L2B9, L2H7,L2H8,L3B12 and L3E11 had a lot of tiny crests and tiny folds, but difference in density, L2C9 had abundant microvilli and L3F9 had massive prominency. DNA contents were 8.8,8.3,8.3,8.5,8.6,8.7,8.9,9.0pg for L1210 and seven sub-lines L2H7,L3F9, L2H8,L2C9,L3B12,L2B9, L3E11,respectively. Statistic analysis indicated significant difference ( P <0.01).Main range of the chromosome number of L1210 cells were 62-63, chromosome number of the clonal cells were 48,58,64,66,62,63,58 of L2B9, L2C9, L2H7,L3B12,L3E11,L3F9,L2H8 respectiverly. With immunohistochemistry different clonal cells had different reactions to 10 kinds of antibodies, and the reaction positive rates of clonal cells were higher than L1210 cells.Conclusion Seven sub -linesshoweddifferentproperties.

关 键 词：原代细胞 L1210细胞 克隆细胞 细胞表面 克隆株 阳性率 免疫组化染色法 细胞学特性 性比 染色体数目 

分 类 号：Q344.13[生物学—遗传学] R735.2[医药卫生—肿瘤]