高效液相色谱法测定人血清中氧氟沙星浓度  

Determination of ofloxacin by HPLC in human serum

作　　者：徐珺[1] 吕纹[1] 安玉洁[1] 

机构地区：[1]北京协和医院药剂科,100730

出　　处：《中国医院药学杂志》1993年第12期535-536,共2页Chinese Journal of Hospital Pharmacy

摘　　要：本文建立了反相高效液相色谱法测定人体血清氧氟沙星浓度的方法。该法用Zorbax-ODS(5μ)色谱柱,0.03mol/L 磷酸;乙腈:40%氢氧化四丁铵水溶液(80∶7∶1,v/v)的混合溶液(pH≈2.3)为流动相,荧光检测器(激发波长295nm,发射波长467nm)检测,吡哌酸为内标物。最低检测浓度为1ng/ml,回收率为91.4%～102.8%。A rapid and sensitive quantitative analytical method for determination of ofloxacin in human serum was devel-oped.The method consists of RP-HPLC and fluorimetric detection.The stationary phase was Zorbax-ODS used atroom temperature.The mobile phase consisted of 0.03mol/L phosphoric acid:acetonitrile:tetrabutylammonium hy-droxide(80:7:1,v/v).Pipemidic acid was used as an internal standard.Detection limit was Ing/ml.Recovery ratevaried from 91.4% to 102.8%.

关 键 词：氧氟沙星 高效液相色谱 血药浓度 

分 类 号：R914.1[医药卫生—药物化学]