应用染色体涂染技术检测食管癌细胞系染色体异常  

DETECTION OF CHROMOSOME ABERRATIONS IN ESOPHAGEAL CARCINOMA CELL LINE EC8712 BY CHROMOSOME PAINTING

作　　者：郑文岭[1] 王秀琴 付明 吴旻 

机构地区：[1]广州军区总医院医学实验科 [2]分子肿瘤学国家重点实验室中国医学科学院肿瘤研究所,北京100021

出　　处：《中华医学遗传学杂志》1993年第4期210-212,共3页Chinese Journal of Medical Genetics

基　　金："863"生物技术资助课题;中国医学科学院资助课题

摘　　要：采用染色体涂染技术,用3号染色体特异的探针,检测了食管细胞系EC8712核型的改变,发现EC8712细胞的一对3号染色体有一条明显变短。与常规细胞遗传学分析比较,染色体涂染技术可以确定特殊的染色体,表明该技术将为深入分析肿瘤的分子细胞遗传学改变提供有效的手段。Chromosome painting is a method of fluorescence in situ hybridization(FISH), which by employing a set of chromosome specific DNA probes, paints individual chromosomes and thus makes the specific pair of chromosomes be easily distinguished. We applied this technique to probe the cytogenetic aberration of a human esophageal carcinoma cell line EC8712. It was found that the pair of chromosome 3 could be clearly delineated. Moreover, the painted chromosomes appeared unequal in length in this specific cell line. This demonstrated that chromosome painting might be an effective approach to the phenotypic analysis of tumor molecular cytogenetics.

关 键 词：染色体 原位杂交 涂染 食管癌 

分 类 号：R735.1[医药卫生—肿瘤]