三组蛋白解离液提取变链球菌抗原的实验研究  

Experimental study of extracting the antigen of S. Mutans by three groups of protein Isolation solution.

作　　者：文立亚[1] 岳松龄[1] 蔡美英[2] 高巽坤[2] 张传彬[2] 

机构地区：[1]华西医科大学口腔医学院口腔内科 [2]华西医科大学微生物学教研室

出　　处：《牙体牙髓牙周病学杂志》1993年第2期67-68,共2页Chinese Journal of Conservative Dentistry

摘　　要：本文对三组不同组分的蛋白解离液在不同温度和时间对变链球菌细胞表面蛋白抗原的提取进行了比较。结果显示,1组提取的蛋白抗原浓度最高,2组次之,3组最低。100℃水浴10min、20min提取的蛋白浓度最高,放置37℃、4℃各24h的提取效果较差。统计学分析各组间相差显著(P<0.05)。SDS-PAGE分析,各组蛋白条带位置均一致。但37℃,4℃的蛋白条带不清晰。本研究认为,将巯基试剂,脲素和SDS按一定比例浓度混合使用,其提取蛋白抗原的效果最佳;此外,适当的温度和时间也是获得较高蛋白抗原浓度的重要因素之一。This paper made a comparison of extracting antigen of S. Mutans by three groups of protein isolation solution under different time and temperature. The results indicated that the highest concentration of protein antigen was group 1, the second was group 2 and the lowest was group 3. The highest was under boiling 100℃ 10-20min ,but if at 37℃ , or at 4℃ ,each for 24h,the concentration of protein antigen all lower. SDS-PAGE analysis showed that protein band of various group was similar. We considered that the better effect of extracting protein antigen of S. Mutans was the mixture of proper concentration of mercaptol agent, urea and SDS, and proper time and temperature were also relative important factors.

关 键 词：蛋白解离液 变链球菌 蛋白抗原 抗原 提取 

分 类 号：R780.2[医药卫生—口腔医学] R378.12[医药卫生—临床医学]