人结肠高分化腺癌细胞系THC—8908的建立及其生物学特性  

Establishment and Characterization of a Cell Line THC-8908 from Human Well Differentiated Colon Adenocarcinoma

作　　者：刘洪(阝刍木)[1] 李川[1] 王立梅[1] 杨毅[1] 战忠利[1] 张天泽[1] 

机构地区：[1]天津市肿瘤研究所

出　　处：《中国肿瘤临床》1993年第5期330-333,F003,共5页Chinese Journal of Clinical Oncology

摘　　要：本文报道将手术切除人结肠腺癌肿瘤标本,接种于裸鼠皮下,获可传代移植瘤,再体外培养,建立可连续培养的结肠高分化腺癌细胞系THC-8908,已传至160代。经光学和电镜检查该细胞系具有上皮性恶性细胞特征,放免检测证实具有分泌CEA能力,并观察了其体外生长曲线、分裂指数、软琼脂上集落形成率,染色体分析众数63,基本为亚三倍体,裸鼠皮下移植形成恶性种瘤,与手术标本病理形态相一致,是进行癌变机理研究和实验性基因治疗的良好模型。A serially transplantable tumor model was established by inoculating subcutaneously tumor tissue from the surgical specimen of a male adult patient with colon adenocarcinoma into nude mice (BALB/c nu/nu), whereupon a human colon well differentiated adenocarcinoma cell line THC-8908 was thus obtained. This cell line has been incessantly propagated in vitro for more than 2 years through 160 passages. All the cells were of epithelial type. The doubling time was 25. 4 hours and the mitotic index was 50. 4‰. Electronmicroscopic examination showed that the cells had large nucle- us, large and irregular abundant microvilli and some secretory granules. The chromosome analysis revealed a mode of 63 per cell and the clone forming rate in soft agar was 16. 8%. CEA was positive in the cells and in the culture supernatant, Alcian blue staining was positive in the cytoplasm. When heterotransplanted to nude mice, the cells grew to form tumor with the same morphology as the original one from the patient. Detection for mycoplasma in the THC-8908 cell line cul- ture was negative.

关 键 词：结肠肿瘤 高分化 腺癌 细胞系 

分 类 号：R735.350.3[医药卫生—肿瘤]