甲基丙烯酸环氧丙酯对金仓鼠胚胎细胞基因表达的影响  

EFFECT OF GLYCIDYL METHACRYLATE(GMA)ON GENEEXPRESSION WITH IN GOLDEN SYRIAN HAMSTER EM-BRYO(SHE)CELLS

作　　者：杨俊保[1] 谢大英[1] 左瑾 许建宁[1] 方福德[1] 

机构地区：[1]北京中国预防医学科学院劳动卫生与职业病研究所,中国医学科学院基础医学研究所

出　　处：《癌变．畸变．突变》1994年第1期6-10,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金

摘　　要：本实验采用Ｎｏｒｔｈｅｒｎ印迹杂文方法研究了甲基丙烯酸环氧丙酯（ＧＭＡ）对金仓鼠胚胎（ＳＨＥ）细胞原癌基因Ｃ─ｍｙｃ和重要功能基因谷胱甘肽Ｓ─转移酶ｐ（ＧＳＴ─ｐ）表达的影响．结果表明ＧＭＡ促进了这些基因的表达，其中ＧＳＴ─ｐ基因在作用２ｈ表达即见增高，５ｈ后达到高水平，直至１２ｈ仍维持这一高水平；ｃ─ｍｙｃ基因在１２ｈ后出现高水平．本文讨论了ＧＭＡ诱导上述基因表达的改变与细胞转化之间的可能关系．To explore preliminaryly the mechanism of golden Syrian hamster embryo(SHE)cells transformation induced by glycidyl methacrylate(GMA),the transeriptionalactivities of some singificant genes such as protooncogene myc(c─myc)and glutathioneS─transferase─p(GST─p)in the SHE cells treated with GMA were examined by usingNorthern blot hybridization with corelative labelled probes。It was observed that the ex-pression level of GST─p m RNA began to increas at 2 hours,and reached to a high steadylevel from 5 hours to 12 hours after the SHE cells were trcated with GMA. The expressionof protooncogene myc reached a peak at l2 hours after the SHE cells treated with GMA.The elevation of the transcription of the genes showed that the regulation of expression ofthe cell genes might be effected by the interference of GMA. We concluded that the activation of expression of c─myc and GST─p gene is important and an initiation step dur- ing the transformation of SHE cells by GMA was discussed.

关 键 词：甲基丙烯酸 环氧丙酯 原癌基因 

分 类 号：R730.231.1[医药卫生—肿瘤]