丙型肝炎病毒携带者染色体不稳定性研究  

STUDY ON CHROMOSOME INSTABILITY IN CARRIERS WIIH HEPATITIS C VIRUS(HVE )

作　　者：李明烈[1] 程俊[1] 毛远萍 

机构地区：[1]上海市肿瘤研究所

出　　处：《癌变．畸变．突变》1994年第2期1-4,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：本文选择丙型肝炎病毒携带者和正常健康者各２０例作外周血淋巴细胞的染色体不稳定性研究．接种于添加０．０９８ｍｇ／Ｌ的ＴＣ１９９培养液的２组血标本进行（Ｃｈｒｏｍｏｓｏｍｅａｂｅｒｒａｔｉｏｎｒａｔｅｓ，ＣＡＲ）和（ｆｒａｇｉｌｅｓｉｔｅｒａｔｅ，ＦＳＲ）观察，结果发现丙型肝炎病毒携带者和正常人的ＣＡＲ分别为３．７０±１．４４和１．３５±１．５０；它们的ＦＳＲ分别为３．１％和１．０％．２组间的ＣＡＲ和ＦＳＲ皆有显著的统计学差异（Ｐ＜０．００１）．接种于添加９ｍｇ／ＬＢｕｄＲ的１６４０培养液的２组血标本作ＳＣＥ分析，结果显示丙型肝炎病毒携带者的ＳＣＥ值（０．１５２±０．０２６）也显著高于正常健康者（０．１０２±０．０１８）．（Ｐ＜０．００１）．我们的初步研究表明丙型肝炎病毒携带者具有明显的染色体不稳定性，并提示丙型肝炎病毒与原发性肝细胞癌的发生具有重要的相关性。The chromosome instability of peripheral lymphocytes was studied in 20 carrierswith hepatitis c virus (HCV)and 20 normal subjects(NS) as controls.The chromosomeaberration rates (CAR) and the fragile site ratc (FSR) in two gronps were determined byuse of TC 199 medium containing 5% fetal bovine serum and 0.098mg / L FUdR. TheCAR were found to be 3.70±1.44and 1.35± 1.50 in carriers with HCV and NS,respectively, with significant statistica1 differences between them(P<0.001).The FSR were3.1% and 1.0% in carriers with HCV and NS, respectively,a1so show significatnt statisticaldefferences between them(P <0.001).Thc frequencies of sister chromtid exchange (SCE)intwo groups were determined by use 1640 medium and 9mg/ L BUdR .The results showedthat the SCE in carriers with HCV(0.152 ± 0.026) were significantly higher than that of theNS(0.102 ± 0.018)(P<0.001). According to our preliminary study, we suggests that there is a significant chromosomeinstability in carricrs with HCV. this may be show that HCV are important associated withhepatocarcinogenesis.

关 键 词：丙型肝炎病毒 肝细胞癌 染色体 

分 类 号：R735.702[医药卫生—肿瘤]