三种毒物对小鼠受精卵染色体畸变的影响  

THE EFFECTS OF THREE GENOTOXIC CHEMICALS ONTHE CHROMOSOMES IN MOUSE ZYGOTES

作　　者：于永强[1] 李修山 李庶良 高敏[1] 邵华[1] 施建安 马国勋 代新洲 张秀珍[1] 

机构地区：[1]青岛化工部化工劳动保护研究所,青岛市化工职业病防治院

出　　处：《癌变．畸变．突变》1994年第5期6-8,共3页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：本文采用小鼠受精卵染色体分析方法，对敌枯双、重铬酸钾、二硫化碳三种毒物的生殖细胞遗传物质诱变作用进行了评价，结果证明阴性对照组染色体数目畸变细胞率为９．Ｏ％，与各染毒组比较均有显著差异，敌枯双组为５１．８％，（Ｐ＜０．０１）；重铬酸钾组为３３．８％，（Ｐ＜０．０１）；二硫化碳（１０ｍｇ／ｍ３）组为２２．８％，（Ｐ＜０．０１），（１００ｍｇ／ｍ３）组为２｝８％，（Ｐ＜０．０１）。In this paper,a new method for chromosome preparetion of zygotes from mouse was described.Mutagenesis of N, N-methyoer -bis (2-amiog-1,3,4,-thiadiazole)(MATDA),Potassium dichromate(PD),Carbon disulflde(CD )damaging tochromosomes in the zygotes was eval uated hy the method.The result shown that theincidence of zyilotes with abnormalities of chromosomal numbers of control group was 9.0%,The incitience of four groups exposed to MATDA( 2mg/kg,wt),PD(4. 8mg/kg.wt), CD(10mg/m3) CD(100mg/m3)were 5l.8%(P<0.01),33.8%(P<0.01),22.8%(P<0.01),29.8%(P<0. 01)respectively,suggested that the genotoxic cliemicals aremutagenic effects on chromosomes of mouse zygotes.

关 键 词：敌枯双 重铬酸钾 二硫化碳 染色体畸变 受精卵 

分 类 号：Q319.31[生物学—遗传学] R114[医药卫生—卫生毒理学]