通过增强癌细胞内自由基反应抗癌的研究  

POTENTIALITY OF DESTRYING TUMOR BY ENHANCING FREE RADICAL REACTIONS IN TUMOR CELLS

作　　者：莫简[1] 孙淑芬[1] 王多宁[1] 郭正仁 孙存普[1] 王志强[1] 晏培松[1] 

机构地区：[1]西安第四军医大学自由基生物学实验室,北京军事医学科学院放射医学研究所,西京医院

出　　处：《癌症》1994年第4期297-298,321,共3页Chinese Journal of Cancer

基　　金：国家自然科学基金

摘　　要：本实验研究了二乙基二硫代氨基甲酸钠（ＤＤＣ）一血卟啉衍生物（ＨＰＤ）—激光及ＤＤＣ—放射线的抗癌作用。其结果表明，ＤＤｃ可显著地提高ＨＰＤ—激光的抗癌作用，使癌细胞死亡率从１５．９％提高到９２．９％（Ｐ＜０．０１）。对荷瘤小鼠，先给ＤＤＣ也可提高其疗效。用ＥＳＲ法，自旋捕捉剂与自由基清除剂研究的结果证明，ＤＤＣ提高ＨＰＤ—激光的抗癌作用与增强癌细胞内自由基反应有关。ＤＤＣ亦可提高γ射线的癌细胞（Ｓ１８０）的杀伤作用，也能提高电子直线加速对小鼠（ＢＡＬＢ／Ｃ）ＥＭＴ６／Ｋｕ实体瘤的疗效。We have studied the antitumor effects of DDC-HPD-laser and DDC-ionizing radition. In invitro studies, the death rate of tumor cells(H22)teated with DDC-HPD-laser was 92.2%.Whereas that of the cells treated with HPD-laser was on only 15.9%. These results show that DDC can significantly enhance the tumor cell-killing effect of HPD-laser (P<0.01 ).In invivo studies,the preliminary result indicates that the treatment with DCC-HPD-laser may be beneficial to tumor-bearing animals. The free radicals in tumor cell suspensions were trapped by DMPO and detected by ESR spectra.The role of free redicals was observed with free radical scavengers. The results of these studies shoW that the enhancement of antitumor effects of HPD-laser by DDC resulted from the enhancement of free radical reactions in tumor cells. Instead of HPD -laser we also used ionizing radiation to generate oxyge-derived radicals and found that DDC also enhanced the killing effect of γ-ray upon tumor cell(S180) and the antitumor effect of ionizing radiation produced by linear accelerator upon transplant EMT6/ku tumor on BALB/cmice.

关 键 词：肿瘤 血卟啉 激光 自由基 

分 类 号：R730.5[医药卫生—肿瘤]