生物素标记探针原位DNA分子杂交中的假阳性  

THE FALSE-SIGNALS IN DNA-DNA IN SITU HYBRIDIZATION WITH BIOTINYLATED PROBE

作　　者：蒋晓群[1] 姚开泰[1] 

机构地区：[1]湖南医科大学肿瘤研究所

出　　处：《癌症》1994年第4期343-345,共3页Chinese Journal of Cancer

基　　金：国家八五计划;中华医学基金会(CMB)和湖南省卫生厅资助课题

摘　　要：以生物素标记的ＥＢ病毒ＢａｍＨＩ酶切ｗ片段为探针，原位检测人鼻咽癌等石蜡切片中的ＥＢ病毒ＤＮＡ，结果发现：在部分标本的癌细胞胞浆中和非癌的鼻咽增生上皮细胞中，鼻咽粘膜下小腺体及唾液腺细胞浆中，存在内源性的亲和素结合活性，因此产生假阳性信号。文章介绍了设立相邻切片的无探针对照来区分真伪的方法，并对生物素标记探针原位杂交技术的适用范围进行了讨论。In the experiment of in situ detection of EBV-DNA on routine paraffin sections of nasopharyngeal carcinoma biopsies’it was discovered that some cells in some biopsies showed endogenous avidin binding activity which resulted in pseudo-signals.The method used to distinguish the false and true positive signals was introduced and the applicable scope of biotinylated probe on in situ hybridization was discussed.

关 键 词：生物素 核酸原位杂交 癌 鼻咽肿瘤 

分 类 号：R739.630.3[医药卫生—肿瘤]