大鼠伏隔核儿茶酚胺能传入纤维的脑干起源—逆行荧光标记法与荧光组化法结合研究  

THE BRAIN STEM ORIGIN OF THE AFFERENT CATECHOLAMINERGIC FIBRES OF THE NUCLEUS ACCUMBENS IN THE RAT—COMBINED RETROGRADE FLUORESCENT LABELLING AND FLUORESCENT HISTOCHEMISTRY STUDY

作　　者：张德兴[1] 覃盛麟 檀进发[1] 柯铭华 

机构地区：[1]广西医学院人体解剖学教研室

出　　处：《神经解剖学杂志》1989年第1期97-102,共6页Chinese Journal of Neuroanatomy

摘　　要：应用逆行荧光标记与Faglu荧光组化相结合技术,探索了大鼠伏隔核CA能传入纤维的脑干起源。大鼠52只,分三组进行实验。单侧注射组,分别将荧光素PI、DAPI或Bb注入一侧伏隔核后,灌流前作优降宁和L-多巴胺腹腔注射,继用Faglu程序处理切片。结果在同侧的A_(10)、A_9、A_8、A_6、A_2和A_1中,发现为荧光素逆行标记的CA神经元;在对侧的A_(10)、A_9、A_2和A_1中,亦见少量的上述神经元。在以PI-Bb或PI-DAPI配对的双侧注射组,在A_(10)和A_9群中可见少量荧光逆行双标记的CA神经元。表明伏隔核除主要接受同侧脑干A_(10)、A_9、A_8、A_6、A_2和A_1的CA传入纤维外,尚接受对侧A_(10)、A_9、A_2和A_1的少量CA传入纤维。此外,在A_(10)和A_9中,存在着少量向双侧伏隔核发出分叉投射的CA神经元。Retrograde fluoresent-labelling combined with the Faglu method has performed in 52 rats to demonstrate the origin in the brain stem of the afferent catecholaminergic fibres of the nucleus accumbens (acb).In the ipsilateral group, fluorescence PI, DAPI or Bb was injected individually into acb. The animals survived for 2 1/2-3 days (for PI, DAPI) or 12—15 hours (for Bb). Before perfusion via the left cardiac ventricle with Faglu solution, Parpyline and L-Dopa for enhancing CA fluorescence was injected intraperitonealiy. The retrograde fluorescent-labelled neurons showing CA fluorescence were traced under Olympus fluorescence microscope in dried frozen serial sections of the brain stem. The results were as follows: 1. Ipsilaterally the retrograde fluorescent-labelled neurons showing CA fluorescence were found in A10, A9 A8, and A6, A2, A1; 2. Contralaterally a few of the labelled neurons were also found in A10, A9, A2, A1.In the bilateral group, PI was injected into the left acb and Bb or DAPI was injected into the opposite acb. A few of the retrograde double fluorescent-labelled neurons showing CA fluorescence were found in A10 and Ag.It is indicated that besides receiving primarily the afferent fibres of CA neurons in the ipsilateral A_(10), A_9, A_8, A_6, A_2, A_1, acb also receives a few of the fibres from the contralateral A_(10), A_9, A_2, A_1. In addition, a few of the CA neurons in A_(10), A_9 project collateral fibres to acb of both sides.

关 键 词：伏隔核 传入纤维 儿茶酚胺 神经元 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]