SCID小鼠几个特性的检测及其初步应用  

Some Characterizations and Tentative Applications of the Scid Mouse

作　　者：范文平[1] 李冠民[1] 黎绍明[1] 关伟红[1] 王秀清[1] 

机构地区：[1]中国药品生物制品检定所

出　　处：《北京实验动物科学与管理》1994年第4期12-14,共3页

摘　　要：我所于１９９３年６月将原饲养在北京实验动物中心的整个ｓｃｉｄ小鼠种群引人，目前生长繁殖状态良好。我们建立了组织学、生理学和免疫学等监测方法，对ｓｃｉｄ小鼠特性进行检测。４～８周龄小鼠，胸腺和脾脏相对重量小，外周血液中白细胞含量少，组织学观察胸腺无皮质结构，髓质保留，缺乏淋巴细胞，脾脏白髓内基本没有淋巴细胞，Ｔ淋巴细胞转化实验中，转化率为零，免疫电泳未监测出免疫球蛋白的存在，这些低龄小鼠渗漏率为１３．２％。３５周龄以上的高龄小鼠中，大部分个体免疫功能有所恢复，渗漏率达７１．４％。同时，我们对ｓｃｉｄ小鼠的应用也作了一些有益的探索。Scid mouse is homozygous for a recessive mutation at a locus thatinfluences the assembly of intact immunoglobulin and T cell receptorgenes. Therefore,scid mice cannot generate functional B and Tlymphocytes,and profoundly immunodeficient, and are valuable animal tostudy turnour.immunology,infections with pathogens and reproductivebiology. In the present study,we have surveied some characterization ofthe scid mice.The scid mice were raised in plastic film isolators inwhich SPF condition was kept.Average litter size of the scid mice was5.6.weaned-to-born ratio was 90%. By 4 to 8 weeks of age, reiativeweigts of the thymus and the spleen were 0.0433 ± 0.0l9l%,0. 0688 ± 0.0l37%respectively The size of the leukocytes per mm3 peripheral blood was 2000±339, In which the lymphocytes were 14～27% .The thymus wasfound to consist of a rudimentary medulla withour cortex and contained few lymphocytes. The spleen follicles were empty virtually devoid of lym- phocytes. occupied by reticular cell.Sera from scid mice have notbeen assayed for the presence of immnuoglobulin. The leaky ratio ofthisyoung scid mice was l 3.3%. By 35 and over weeks of age ,the majority of scid mice were leaky, and its ratio was 7l.4%. Our works will be of great value to breeding.management and quality control of scid mice. the tentative apptications of scid mice were made in this study too。

关 键 词：SCID小鼠 特性 应用 实验动物 动物模型 

分 类 号：Q95－331[生物学—动物学] R－332[医药卫生]