β－受体激活对血小板激活因子、组胺致血管壁通透性增高的抑制作用  

The Inhibitory Effects of β-Adrenoceptor Activation on Vascular Permeability Increment Induced by Platelet-Activating Factor and Histamine

作　　者：蒋美诊 李少华[1] 张亚霏[1] 丁自强[1] 

机构地区：[1]第二军医大学基础医学部病理生理学教研室

出　　处：《第二军医大学学报》1994年第3期207-211,共5页Academic Journal of Second Military Medical University

摘　　要：本实验采用整体动物和体外灌注内皮细胞单层模型，研究了异丙肾上腺素（ＩＰＮ）对炎症介质引起的血管壁通透性增加的影响。结果表明，大鼠静注伊文斯蓝２０ｍｇ／ｋｇ后，皮内注射１０￣（－７）ｍｏｌ／ＬＰＡＦ或０．１ｍｇ／ｍｌ组胺０．２ｍｌ，分别引起局部伊文斯蓝渗出１６．８２±２．０５μｇ／ｇ组织和２１．６８±２．８６μｇ／ｇ组织。预先静注ＩＰＮ１０μｇ／ｋｇ，伊文斯蓝渗出量分别减少５６．２％和４３．６％。心得安（ＰＰＮ）可逆转ＩＰＮ的作用。单纯心得安处理，使ＰＡＦ和组胺引起的伊文斯蓝渗出量增高，提示生理浓度的儿茶酚胺对血管壁通透性有抑制作用。体外灌注内皮细胞单层研究表明，ＩＰＮ可显著降低ＰＡＦ引起的跨内皮单层液体流量和蛋白清除率的增高，从细胞水平证实ＩＰＮ可抑制ＰＡＦ引起的血管壁通透性增高。The effects of isoproterenol(IPN) on histamine and PAF-induced vascular permeability increment in rat skin and in cultured confluent endothelial cell monolayers were investigated.The results showed that after intravenous administration of 20 mg/kg Evans blue(EB) dye,intradermal injection of 0.2ml PAF(1O-7 mol/l) or histamine(0.1 mg/ml) induced 16.82±2.05 μg/g tissue and 21.86±2.86 μg/g tissue EB exudation respectively in the injected skin.Pretreatment of the rats with intravenous IPN(10 μg/kg) decreased EB exudation 56.2% and 43.6% respectively.Propranolol (PPN),a β-adrenoceptor blocker,reversed the inhibitory effects of IPN.In those rats treated with PPN alone,intradermal injection of PAF and histamine induced significant increase of skin EB exudiltion in comparison with the control,indicating that catecholamines at physiologic concentration in vivo may have inhibitory effects on vascular permeabity.When cultured endothelial cell monolayers were perfused with 1% albumin in Hanks,balanced salt solution(HBSS),the increased fluid flux and protein clearance caused by PAF were significantly decreased by IPN pretreatment.The results confirmed the inhibitory effects of IPN on vascular permeability increment induced by PAF at the cellular level.

关 键 词：血小板 激活因子 组胺 心得安 

分 类 号：R364.5[医药卫生—病理学]