蝌蚪提取液对小鼠红白血病细胞的影响  

Influence of extract from tadpoles(T-871) on murine erythroleukemia cells

作　　者：白经修[1] 丁蔚[1] 张娓[1] 王英华[1] 牛富文 

机构地区：[1]河南医科大学组织胚胎学教研室

出　　处：《河南医科大学学报》1994年第4期337-340,共4页Journal of Henan Medical University

基　　金：河南省教委资助

摘　　要：以２％的蝌蚪提取液培养小鼠红白血病细胞（ＭＥＬＣ）。结果：生长曲线显示蝌蚪提取液可抑制ＭＥＬＣ生长；联苯胺染色反应显示ＭＥＬＣ胞浆中血红蛋白的合成随提取液作用时间的延长而增加，说明蝌蚪提取液有诱导ＭＥＬＣ发生终未分化的作用；流式细胞仪分析证明蝌蚪提取液可造成Ｇ０和Ｇ１期细胞堆积，Ｇ２和Ｍ期细胞减少。另经ＳＤＳ－聚丙烯酰胺凝胶电泳分析：该提取液中的有效作用物质是一组分子量７万Ｄａ左右的肽类，在本提取液中的含量为４．５ｇ／Ｌ。结果提示：蝌蚪提取液起诱导作用的成分易纯化，可能成为研究肿瘤恶性逆转的良好诱导分化剂。The murine ervthroleukemia cells(MELCs) cultured with the extract[1:50(v/v)concentration]from tadpoles(T-871) were studied in this experiment.The results showed that T-871 could inhibit the growth of the MELCs and the reaction stained with benzidine hydrochloride showed that the amount of hemoglobin synthesized was increased as the exposure time lengthened.T-871 could induce MELCs terminal differentiation.The results analyzed with flow cvtometry(FACS-420)demonstrated that the number of cells accumulated at G0+G1 phases and the number of cells decreased at G2 +M phases.The SDS-PAGE showed that a group of molecular weights of T-871 in effect on MELJCs were about 70 000 dalton and its chemical nature was of peptide.The effective contents of T-871 were 4.5 g/L. In our study,it was suggested that the effective components induced by T-871 be easily purified and could become good inducer for research on malignant cell reversion.

关 键 词：蝌蚪 提取液 红白血病 细胞 

分 类 号：R733.730.5[医药卫生—肿瘤] R73－36[医药卫生—临床医学]