Ras癌基因转化的肝细胞系的生长和分化特性研究  

Transformation of a SV_(40)-immortalized Hepatocyte Cell Line by Ras without Loss of Expression of Certain Liver-specific Genes

作　　者：孟运莲[1] 袁华[1] 

机构地区：[1]湖北医科大学

出　　处：《湖北医科大学学报》1994年第3期199-201,共3页

摘　　要：用活化的Ｃ－Ｈａ－Ｒａｓ基因转染一株ＳＶ４０转化的肝细胞系──ＣＷＳＶ１细胞，形成了稳定的转化细胞（ＮＲ细胞）。检测了Ｒａｓ癌基因对转化细胞的生长和分化特性的作用。我们的结论是：（１）活化的Ｒａｓ基因转入高度分化的大鼠肝细胞系能显著地改变其生长特性和致瘤潜能，但不改变某些与肝细胞分化特性有关的基因的表达；（２）Ｒａｓ转化细胞的成瘤性和它们的不贴壁生长有关；（３）Ｒａｓ基因的转化没有诱发转化细胞的甲胎球蛋白基因的表达。We transfected the activated C-Ha-Ras DNA into a low passage SV40-immortalized rat hepatocyte cell line, CWSV1, and established stable transfectants. The growth properties and differentiation of Ras transfectants were examined. We conclude that the addition of the activated Ras oncogene to CWSV1 cells can alter the growth properties and tumorigenicity of these cells without alteririg their ablity to express certain liver-specific genes. The tumorigenic potential of the Ras transfectants is related to their anchorage indepehdent growth.

关 键 词：致癌基因 转化 遗传 细胞分化 

分 类 号：R730.231.3[医药卫生—肿瘤]