外源DNA完全酶解成分在水稻浸胚法导入中的诱变作用  

THE MUTATION-INDUCING FUNCTION OF COMPLETELY DIGESTED DNA DURING THE EMBRYO-SOAKING INTRODUCING OF EXOGENOUS DNA INTO RICE

作　　者：张学文[1] 唐湘如[1] 董延瑜[1] 

机构地区：[1]湖南农业大学生物技术系,湖南农业大学农学系

出　　处：《湖南农学院学报》1994年第6期535-537,共3页

基　　金：湖南省自然科学基金

摘　　要：外源ＤＮＡ以ＤＮＡ酶（ＤＮａｓｅⅠ）完全酶解后，浸泡处理灿型中优早２号糙米，研究了外源ＤＮＡ酶解成分在水稻浸胚法导入中的诱变作用。处理当代即表现了一定程度的变异，表明浸胚法外源ＤＮＡ导入过程中确存在着完全降解成分的诱变作用。The mutation-inducing function of the elements of the completely digested DNA treated with nuclease(DNase 1) was studied by means of soaking the rice grains of Zhongyouzao 2 with the digested DNA solution.Mutations found at some extant in the soaked generation.It showed that some mutations were induced by the elements of complete-ly digested DNA during embryo-soaking introducing of foreign DNA into rice.

关 键 词：外源 脱氧核糖核酸 水稻 浸泡法 酶水解 诱变 

分 类 号：S511.103.2[农业科学—作物学]