原发性肝癌组织HBVDNA存在状态及HBsAg、HBcAg表达的检测  

The Status of HBV DNA and Expressions of HBsAg and HBcAg in Primary Hepatocellular Carcinoma

作　　者：周凤[1] 王锦蓉[1] 雷秉钧[1] 赵连三[1] 唐红[1] 王小飞[1] 刘丽[1] 刘聪[1] 穆仁懋[1] 

机构地区：[1]华西医科大学附属第一医院传染内科

出　　处：《华西医学》1994年第4期419-421,共3页West China Medical Journal

摘　　要：本文采用Ｓｏｕｔｈｅｒｎ印迹杂交技术检测原发性肝癌（ＰＨＣ）组织ＨＢＶＤＮＡ存在状态，同时应用免疫组化染色检测ＰＨＣ组织内ＨＢｓＡｇ、ＨＢｃＡｇ表达，并比较ＨＢｓＡｇ、ＨＢｃＡｇ在ＰＨＣ组织与癌旁肝组织的定位分布。结果显示：整合型ＨＢＶＤＮＡ在３２例ＰＨＣ组织检出率７１．９％（２３／３２），癌旁组织检出率４０．７％（１１／２７）；ＰＨＣ组织内检出ＨＢＳＡｇ阳性细胞１２例（３７．５％）未检出ＨＢＣＡｇ；癌组织检出ＨＢｓＡｇ２６例（９６．３％），ＨＢｃＡ９５例（１８．５％）。结果提示：ＨＢＶ感染与ＰＨＣ发生密切相关，ＨＢＶ可能通过整合于宿主细胞染色体对ＰＨＣ发生起一定作用。cases of surgical specimens with PHC were studied to delect the status of HBVDNA and expressions of HBsAg and HBcAg by Southern blot hybridization and immunohistochemical methods respectively. The location and distribution of HBsAg and HBcAg in tumors were compared with those in para-tumorous liver tissues. The results showed that the positive rate of integrated HBV DNA was 71.9% (23/32)in tumors and 40.7% (11/27) in the non-tumors respectively. There were 12 cases(37.5%)positive for HBsAg and none positive for HBcAg in tumors. Twenty-six cases(96.3%) positive for HBsAg and 5 cases positive for HBcAg in non-tumors.They suggested that PHC is closely associated with the infection of HBV.It might be of the integration of HBV DNA into the chromosome of hepatoeytes to take part in the pathogeneses of PHC.

关 键 词：肝肿瘤 癌 乙型肝炎病毒 杂交 免疫组织化学 

分 类 号：R735.703[医药卫生—肿瘤]